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Evaluation of growth and motility in non-photosynthetic Azospirillum brasilense exposed to red, blue, and white light
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-02-20 , DOI: 10.1007/s00203-020-01829-8
Molina Romina 1 , López Gastón 1 , Rodríguez Belén 1 , Rosas Susana 1 , Mora Verónica 1 , Cassán Fabricio 1
Affiliation  

Azospirillum brasilense is a non-photosynthetic rhizobacterium that promotes the growth of plants. In this work, we evaluated the effects of different light qualities on the growth, viability, and motility in combination to other culture conditions such as temperature or composition of the culture medium. Exponential cultures of A. brasilense Az39 were inoculated by drop-plate method on nutritionally rich (LB) or chemically defined (MMAB) media in the presence or absence of Congo Red indicator (CR) and exposed continuously to white light (WL), blue light (BL), and red light (RL), or maintained in dark conditions (control). The exposure to BL or WL inhibited growth, mostly in LB medium at 36 °C. By contrast, the exposure to RL showed a similar behavior to the control. Swimming motility was inhibited by exposure to WL and BL, while exposure to RL caused only a slight reduction. The effects of WL and BL on plant growth-promoting rhizobacteria should be considered in the future as deleterious factors that could be manipulated to improve the functionality of foliar inoculants, as well as the bacterial effects on the leaf after inoculation.

中文翻译:

暴露于红、蓝和白光的非光合固氮螺菌的生长和运动性评估

Azospirillum brasilense 是一种促进植物生长的非光合根际细菌。在这项工作中,我们结合其他培养条件(如温度或培养基组成)评估了不同光质量对生长、活力和运动性的影响。在存在或不存在刚果红指示剂 (CR) 的情况下,通过滴板法将 A. brasilense Az39 的指数培养物接种到营养丰富 (LB) 或化学成分确定 (MMAB) 培养基上,并持续暴露于白光 (WL)、蓝色光 (BL) 和红光 (RL),或保持在黑暗条件下(控制)。暴露于 BL 或 WL 会抑制生长,主要是在 36°C 的 LB 培养基中。相比之下,暴露于 RL 表现出与对照相似的行为。暴露于 WL 和 BL 会抑制游泳运动,而暴露于 RL 仅导致轻微减少。WL 和 BL 对促进植物生长的根际细菌的影响在未来应该被视为有害因素,可以通过操纵来改善叶面接种剂的功能,以及接种后对叶片的细菌影响。
更新日期:2020-02-20
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