Summary Two species of digenetic trematodes of the genus Pleurogenoides viz., P. cyanophlycti Shinad & Prasadan (2018a) and P. euphlycti Shinad & Prasadan (2018b) have been described from India. Information regarding the molecular data of various species of the genus Pleurogenoides Travassos, 1921 is virtually lacking. This study addresses the application of molecular markers to validate the phylogenetic position of P. cyanophlycti and P. euphlycti. In the present study, two species P. cyanophlycti and P. euphlycti were collected between January 2016 to October 2017, infecting the freshwater frogs inhabiting the Western Ghats, India. In the present study, the two species were identifi ed morphologically and by PCR amplification of the 28S ribosomal RNA gene. Phylogenetic tree results clearly demonstrate that both P. cyanophlycti and P. euphlycti belongs to the family Pleurogenidae Looss, 1899. Based on these results, we presented and discussed the phylogenetic relationships of P. cyanophlycti and P. euphlycti within family Pleurogenidae from India. Phylogenetic analyses showed that P. cyanophlycti and P. euphlycti cluster according to their vertebrate host and revealed an important congruence between the phylogenetic trees of Pleurogenoides and of their vertebrate hosts. P. cyanophlycti and P. euphlycti clearly constitute a separate, sister branch with other species of the genera, Pleurogenoides, Pleurogenes (=Candidotrema), Prosotocus and Brandesia. The present study firstly provides important information about the molecular study and phylogenetic analysis of P. cyanophlycti and P. euphlycti. This study will also serve as a baseline for Pleurogenoides species identifi cation for further studies.

中文翻译：

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栖息于印度西高止山脉的印度蠹蛙的十二指肠 Pleurogenoides 物种 (Plagiorchiida: Pleurogenidae) 的系统发育位置, Euphlyctis cyanophlyctis (Amphibia: Dicroglossidae)

总结 已在印度描述了两种 Pleurogenoides 属的双遗传吸虫，即 P. cyanophlycti Shinad & Prasadan (2018a) 和 P. euphlycti Shinad & Prasadan (2018b)。几乎缺乏关于 1921 年 Pleurogenoides Travassos 属的各种物种的分子数据的信息。本研究致力于应用分子标记来验证 P. cyanophlycti 和 P. euphlycti 的系统发育位置。在本研究中，2016 年 1 月至 2017 年 10 月期间收集了两种 P. cyanophlycti 和 P. euphlycti，感染了印度西高止山脉的淡水蛙。在本研究中，通过形态学和 28S 核糖体 RNA 基因的 PCR 扩增鉴定了这两个物种。系统发育树结果清楚地表明 P. cyanophlycti 和 P. euphlycti 属于 Pleurogenidae Looss，1899 年。基于这些结果，我们提出并讨论了 P. cyanophlycti 和 P. euphlycti 在来自印度的 Pleurogenidae 科中的系统发育关系。系统发育分析表明 P. cyanophlycti 和 P. euphlycti 根据它们的脊椎动物宿主聚集，并揭示了 Pleurogenoides 和它们的脊椎动物宿主的系统发育树之间的重要一致性。P. cyanophlycti 和 P. euphlycti 显然与该属的其他物种 Pleurogenoides、Pleurogenes (= Candidotrema)、Prosotocus 和 Brandesia 构成一个单独的姐妹分支。本研究首先为P. cyanophlycti和P. euphlycti的分子研究和系统发育分析提供了重要信息。