BACKGROUND Porcine teschovirus (PTV) circulates among wild and domesticated pig populations without causing clinical disease, however neuroinvasive strains have caused high morbidity and mortality in the past. In recent years, several reports appeared with viral agents as a cause for neurologic signs in weanling and growing pigs among which PTV and new strains of PTV were described. CASE PRESENTATION On two unrelated pig farms in the Netherlands the weanling pig population showed a staggering gate, which developed progressively to paresis or paralysis of the hind legs with a morbidity up to 5%. After necropsy we diagnosed a non-suppurative encephalomyelitis on both farms, which was most consistent with a viral infection. PTV was detected within the central nervous system by qPCR. From both farms PTV full-length genomes were sequenced, which clustered closely with PTV-3 (98%) or PTV-11 (85%). Other common swine viruses were excluded by qPCR and sequencing of the virus. CONCLUSION Our results show that new neuroinvasive PTV strains still emerge in pigs in the Netherlands. Further research is needed to investigate the impact of PTV and other viral agents causing encephalomyelitis within wild and domestic pig populations supported by the awareness of veterinarians.

中文翻译：

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在荷兰，有两种新颖的猪破伤风病毒作为脑脊髓炎的病原体。

背景技术猪破伤风病毒（PTV）在野生和驯养的猪群中循环传播而不会引起临床疾病，但是神经侵染株过去已引起高发病率和高死亡率。近年来，关于断奶和生长中猪的神经系统症状的原因，病毒制剂出现了一些报道，其中描述了PTV和新的PTV株。病例介绍在荷兰的两个无亲缘关系的养猪场中，断奶的猪群显示出交错的门，逐渐发展为后腿轻瘫或瘫痪，发病率高达5％。尸检后，我们在两个农场均诊断出非化脓性脑脊髓炎，与病毒感染最一致。通过qPCR在中枢神经系统内检测到PTV。从这两个农场的PTV全长基因组进行了测序，与PTV-3（98％）或PTV-11（85％）紧密结合。通过qPCR和病毒测序排除了其他常见的猪病毒。结论我们的结果表明，荷兰的猪中仍出现新的神经侵入性PTV株。在兽医意识的支持下，需要进行进一步的研究来调查PTV和其他病毒制剂对野生和家养猪种群引起脑脊髓炎的影响。