The immobilization of lipases in cross linked aggregates (CLEA) leads to a robust biocatalyst that remains very stable in low viscous non-conventional Deep Eutectic Solvents - Buffer mixtures. To reinforce that stability, and to facilitate the biocatalyst recovery, this paper explores the immobilization of Lipase-CLEA derivatives in Lentikats®. This double immobilization can be successfully used in esterifications in DES-buffer media, using substrates of unpaired solubilities (e.g. benzoic acid and glycerol), in batch and continuous processes, and reaching full conversion. Under these conditions, the derivatives display an improved stability (compared to the Lipase-CLEA derivatives) and enable the reuse of the reaction media in continuous devices for at least 6 cycles under non-optimized conditions, accumulating 10 g product L-1, enhancing the productivity, and opening exciting future options for sustainable chemistry.

中文翻译：

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封装在lentikats®中的固定化脂肪酶-CLEA聚集体，作为在深共熔溶剂中连续过程的可靠生物催化剂。

脂肪酶在交联聚集体中的固定化（CLEA）导致了一种健壮的生物催化剂，该催化剂在低粘度非常规深层共晶溶剂-缓冲液混合物中保持非常稳定。为了增强稳定性，并促进生物催化剂的回收，本文探讨了Lentikats®中脂肪酶-CLEA衍生物的固定化。这种双重固定可以成功地用于DES缓冲液中的酯化反应中，使用不成对溶解度的底物（例如苯甲酸和甘油），分批和连续进行，并达到完全转化。在这些条件下，衍生物显示出更高的稳定性（与Lipase-CLEA衍生物相比），并且能够使反应介质在非优化条件下在连续装置中重复使用至少6个循环，从而积聚10 g的产物L-1，