We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87 % inside of the same patient cohort for which the markers were developed and up to 81 % in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.

中文翻译：

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评估基于宿主的分子标记物以用于人类败血症的早期检测。

我们已经基于人类基因组中的循环核酸（CNA）鉴定了24种分子标记，这些标记可结合用于实时定量PCR（qPCR）分析中以鉴定人类败血症的存在，从两到三个开始在出现第一个临床体征前几天，包括符合SEPSIS-3标准的患者。在针对其开发标记的同一患者队列中，其准确度超过87％，在对未包括在标记开发中的患者队列的盲法研究中，其准确性高达81％。由于我们的标记物是基于宿主的，因此它们可用于捕获细菌和真菌性败血症，这与当前基于PCR的测试不同，后者需要对引起人类败血症的每种生物都需要特定种类的引物。