The relevant microorganims driving efficiency changes in anaerobic digestion of phenol remains uncertain. In this study correlations were established between microbial population and the process performance in an anaerobic sequencing batch reactor (ASBR) treating increasing concentrations of phenol (from 120 to 1200 mg L-1). Sludge samples were taken at different operational stages and microbial community dynamics was analyzed by 16S rRNA sequencing. In addition, bamA gene was quantified in order to evaluate the dynamics of anaerobic aromatic degraders. The microbial community was dominated by Anaerolineae, Bacteroidia, Clostridia, and Methanobacteria classes. Correlation analysis between bamA gene copy number and phenol concentration were highly significant, suggesting that the increase of aromatic degraders targeted by bamA assay was due to an increase in the amount of phenol degraded over time. The incremental phenol concentration affected hydrogenotrophic archaea triggering a linear decrease of Methanobacterium and the growth of Methanobrevibacter. The best performance in the reactor was at 800 mg L-1 of phenol. At this stage, the highest relative abundances of Syntrophorhabdus, Chloroflexus, Smithella, Methanolinea and Methanosaeta were observed and correlated positively with initial degradation rate, suggesting that these microorganisms are relevant players to maintain a good performance in the ASBR.

中文翻译：

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微生物种群动态，bamA基因丰度与厌氧测序批处理反应器（ASBR）处理日益增加的苯酚浓度之间的相关性。

苯酚厌氧消化中有关微生物的驱动效率变化仍然不确定。在这项研究中，建立了微生物种群与厌氧排序批处理反应器（ASBR）中处理酚浓度（从120到1200 mg L-1）的过程性能之间的相关性。在不同的操作阶段采集污泥样品，并通过16S rRNA测序分析微生物群落动态。另外，定量bamA基因以评估厌氧芳族降解剂的动力学。微生物群落主要由厌氧菌，细菌，梭状芽胞杆菌和甲烷菌组成。bamA基因拷贝数与苯酚浓度之间的相关性分析非常显着，这表明bamA分析靶向的芳香族降解剂的增加是由于苯酚降解量随时间的增加而增加。苯酚浓度的增加影响了氢营养性古细菌，从而触发了甲烷细菌的线性减少和甲烷短杆菌的生长。反应器中的最佳性能是在800 mg L-1的苯酚。在此阶段，观察到最高的Syntrophorhabdus，Chloroflexus，Smithella，Methanolinea和Methanosaeta相对丰度，并且与初始降解率呈正相关，表明这些微生物是维持ASBR良好性能的重要因素。苯酚浓度的增加影响了氢营养性古细菌，从而触发了甲烷细菌的线性减少和甲烷短杆菌的生长。反应器中的最佳性能为800 mg L-1的苯酚。在这个阶段，观察到最高的Syntrophorhabdus，Chloroflexus，Smithella，Methanolinea和Methanosaeta相对丰度，并且与初始降解率呈正相关，表明这些微生物是维持ASBR良好性能的重要因素。苯酚浓度的增加影响了氢营养性古细菌，从而触发了甲烷细菌的线性减少和甲烷短杆菌的生长。反应器中的最佳性能为800 mg L-1的苯酚。在这个阶段，观察到最高的Syntrophorhabdus，Chloroflexus，Smithella，Methanolinea和Methanosaeta相对丰度，并且与初始降解率呈正相关，表明这些微生物是维持ASBR良好性能的重要因素。