Silent oestrus is an unsurmountable problem in the management of buffalo reproduction. In addressing this issue, we have earlier reported variation in the levels of urinary luteinizing hormone (LH) through the different phases of oestrous cycle with an extended window during the mid-oestrous phase. Based on this report, the present study is designed to assess the salivary LH levels in buffalo during the different phases of oestrous cycle. Bovine LH ELISA kit was used to determine the level of salivary LH. We observed a notable variation in salivary LH levels during the different phases of oestrous cycle. The maximum LH level, 39.07 mIU/ml, observed during oestrus, which was significantly (p < .05) higher than other consecutive phases. Altogether, the results showed a significant (p < .05) fold variation during oestrus compared with other phases. Therefore, the study convincingly shows that salivary LH has the potential of application in development of a modality for non-invasive oestrous detection in buffalo.

中文翻译：

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唾液黄体生成激素：检测水牛发情期的敞开窗口。

沉默的发情是水牛繁殖管理中不可克服的问题。在解决这个问题时，我们较早地报道了在雌性周期的不同阶段，尿中黄体生成激素（LH）水平的变化，而在雌性中期，窗口延长了。基于此报告，本研究旨在评估水牛在发情周期不同阶段的唾液LH水平。牛LH ELISA试剂盒用于测定唾液LH水平。我们观察到在雌性周期的不同阶段唾液中LH水平有显着变化。发情期间观察到的最大LH水平为39.07 mIU / ml，比其他连续期显着（p <.05）高。总而言之，结果显示出显着性（p <。05）发情期间与其他阶段相比的倍数变化。因此，该研究令人信服地表明，唾液LH在开发用于水牛的非侵入性雌性检测方法中具有潜力。