当前位置: X-MOL 学术Prostag. Other Lipid Mediat. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Assessment of soluble epoxide hydrolase activity in vivo: A metabolomic approach.
ProstaglandIns & Other Lipid Mediators ( IF 2.9 ) Pub Date : 2020-01-10 , DOI: 10.1016/j.prostaglandins.2020.106410
Darko Stefanovski 1 , Pei-An Betty Shih 2 , Bruce D Hammock 3 , Richard M Watanabe 4 , Jang H Youn 5
Affiliation  

Soluble epoxide hydrolase (sEH) converts several FFA epoxides to corresponding diols. As many as 15 FFA epoxide-diol ratios are measured to infer sEH activity from their ratios. Using previous data, we assessed if individual epoxide-diol ratios all behave similarly to reflect changes in sEH activity, and whether analyzing these ratios together increases the power to detect changes in in-vivo sEH activity. We demonstrated that epoxide-diol ratios correlated strongly with each other (P < 0.05), suggesting these ratios all reflect changes in sEH activity. Furthermore, we developed a modeling approach to analyze all epoxide-diol ratios simultaneously to infer global sEH activity, named SAMI (Simultaneous Analysis of Multiple Indices). SAMI improved power in detecting changes in sEH activity in animals and humans when compared to individual ratio estimates. Thus, we introduce a new powerful method to infer sEH activity by combining metabolomic determination and simultaneous analysis of all measurable epoxide-diol pairs.

中文翻译:

体内可溶性环氧化物水解酶活性的评估:一种代谢组学方法。

可溶性环氧化物水解酶(sEH)将几种FFA环氧化物转化为相应的二醇。据测量,多达15个FFA环氧-二醇比率可从其比率推断sEH活性。使用以前的数据,我们评估了各个环氧-二醇比率是否均表现出相似的行为以反映sEH活性的变化,并且一起分析这些比率是否增加了检测体内sEH活性变化的能力。我们证明环氧-二醇比率彼此之间具有很强的相关性(P <0.05),表明这些比率均反映了sEH活性的变化。此外,我们开发了一种建模方法来同时分析所有环氧-二醇比率,以推断总体sEH活性,称为SAMI(多指标同时分析)。与单个比率估算值相比,SAMI提高了检测动物和人类sEH活性变化的能力。因此,我们通过结合代谢组学测定和所有可测量的环氧-二醇对的同时分析,引入了一种新的强大方法来推断sEH活性。
更新日期:2020-01-10
down
wechat
bug