Alpha-mangostin (α-mangostin) has been identified as a naturally occurring compound with potential anticancer properties. It can induce apoptosis and inhibit the growth and metastasis of cancer cells. Moreover, α-mangostin reduces the mechanical stiffness of lung cancer cells. The objective of this study was to determine the effect of α-mangostin on the mechanical stiffness of various cells, as well as cell viability. The following cell types were examined: human fibroblast TIG-1 cells, human cancerous HeLa cells, human embryonic kidney HEK293 cells, mouse macrophage RAW 264.7 cells, and human myeloblasts KG-1 cells. Cells were treated with α-mangostin, and then examined for cell viability, actin cytoskeletal structures, and surface mechanical stiffness using atomic force microscopy. α-Mangostin demonstrated cytotoxicity against TIG-1, HeLa, HEK293, and KG-1 cells, but not against RAW 264.7 cells. The cytotoxic effect of α-mangostin varies according to cell type. On the other hand, α-mangostin reduced the mechanical stiffness of all cell types, including RAW 264.7 cells. Upon treatment with α-mangostin, F-actin was slightly reduced but the actin cytoskeletal structures were little altered in these cells. Thus, reducing mechanical stiffness of animal cells is an inherent effect of α-mangostin. Our results show that α-mangostin is a naturally occurring compound with potential to change the actin cytoskeletal micro-structures and reduce the surface stiffness of various cells.

中文翻译：

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α-山竹素可降低各种细胞的机械刚度。

Alpha-mangostin (α-mangostin) 已被鉴定为具有潜在抗癌特性的天然化合物。它可以诱导细胞凋亡并抑制癌细胞的生长和转移。此外，α-山竹素可降低肺癌细胞的机械刚度。本研究的目的是确定 α-山竹素对各种细胞的机械刚度以及细胞活力的影响。检查了以下细胞类型：人成纤维细胞 TIG-1 细胞、人癌性 HeLa 细胞、人胚肾 HEK293 细胞、小鼠巨噬细胞 RAW 264.7 细胞和人成髓细胞 KG-1 细胞。用α-山竹素处理细胞，然后使用原子力显微镜检查细胞活力、肌动蛋白细胞骨架结构和表面机械刚度。α-Mangostin 对 TIG-1、HeLa、HEK293 和 KG-1 细胞，但不针对 RAW 264.7 细胞。α-山竹素的细胞毒作用因细胞类型而异。另一方面，α-mangostin 降低了所有细胞类型的机械刚度，包括 RAW 264.7 细胞。在用 α-山竹素处理后，F-肌动蛋白略有减少，但这些细胞中的肌动蛋白细胞骨架结构几乎没有改变。因此，降低动物细胞的机械刚度是α-山竹素的固有作用。我们的研究结果表明，α-山竹素是一种天然存在的化合物，具有改变肌动蛋白细胞骨架微结构和降低各种细胞表面刚度的潜力。包括 RAW 264.7 单元格。在用 α-山竹素处理后，F-肌动蛋白略有减少，但这些细胞中的肌动蛋白细胞骨架结构几乎没有改变。因此，降低动物细胞的机械刚度是α-山竹素的固有作用。我们的研究结果表明，α-山竹素是一种天然存在的化合物，具有改变肌动蛋白细胞骨架微结构和降低各种细胞表面刚度的潜力。包括 RAW 264.7 单元格。在用 α-山竹素处理后，F-肌动蛋白略有减少，但这些细胞中的肌动蛋白细胞骨架结构几乎没有改变。因此，降低动物细胞的机械刚度是α-山竹素的固有作用。我们的研究结果表明，α-山竹素是一种天然存在的化合物，具有改变肌动蛋白细胞骨架微结构和降低各种细胞表面刚度的潜力。