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Comparative Genomic Analysis of a Multidrug-Resistant Campylobacter jejuni Strain YH002 Isolated from Retail Beef Liver.
Foodborne Pathogens and Disease ( IF 2.8 ) Pub Date : 2020-09-02 , DOI: 10.1089/fpd.2019.2770
Sandeep Ghatak 1 , Yiping He 2 , Sue Reed 2 , Peter Irwin 2
Affiliation  

Campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide. In this study, we report the comparative genomic and functional characteristics of C. jejuni YH002 recently isolated from retail beef liver. Whole-genome sequencing and annotation of the strain revealed novel genetic features, including an integrated intact phage element, multiple antimicrobial resistance (AMR) genes, virulence factors, and a Phd-Doc type toxin–antitoxin (TA) system. Phenotypic tests of AMR showed that C. jejuni YH002 was resistant to amoxicillin and tetracycline, which correlates with the AMR genes found in the strain. Comparative analysis of cell motility at genotypic and phenotypic levels identified discernible patterns of amino acid changes, which could explain the variations of motility among C. jejuni strains. Together, these results provide important clues to the genetic mechanisms of AMR and cell motility in C. jejuni. The finding of a Phd-Doc TA system in the genome of C. jejuni YH002 is the first report of this TA system in Campylobacter spp.

中文翻译:

从零售牛肉肝脏中分离的多药耐药空肠弯曲杆菌菌株 YH002 的比较基因组分析。

空肠弯曲杆菌是全世界细菌性胃肠炎的主要原因。在这项研究中,我们报告了最近从零售牛肝中分离的空肠弯曲杆菌YH002的比较基因组和功能特征。该菌株的全基因组测序和注释揭示了新的遗传特征,包括整合的完整噬菌体元件、多重抗微生物 (AMR) 基因、毒力因子和 Phd-Doc 型毒素-抗毒素 (TA) 系统。AMR 的表型测试表明,空肠弯曲杆菌YH002 对阿莫西林和四环素具有抗性,这与该菌株中发现的 AMR 基因相关。在基因型和表型水平上对细胞运动的比较分析确定了可辨别的氨基酸变化模式,这可以解释空肠弯曲杆菌菌株之间运动的变化。总之,这些结果为空肠弯曲杆菌AMR 的遗传机制和细胞运动性提供了重要线索。在空肠弯曲杆菌YH002基因组中发现 Phd-Doc TA 系统是该 TA 系统在弯曲杆菌属中的第一份报告。
更新日期:2020-09-10
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