Long non-coding RNAs (lncRNAs) have been implicated in regulation of biological processes. The role of lncRNAs in macrophages in response to Mycobacterium tuberculosis infection has not been explored. We used high throughput lncRNA microarray analysis to detect differentially expressed lncRNAs and mRNAs in RAW264.7 macrophages with or without M. tuberculosis infection. Quantitative real-time PCR (qRT-PCR) was used to verify the microarray results. Bioinformatics analysis (GO and KEGG) were used to explore the function of significantly dysregulated genes. Microarray results indicated that 1,487 lncRNAs (791 up and 696 down) and 910 mRNAs (536 up and 374 down) were expressed differentially in RAW264.7 macrophages with M. tuberculosis infection compared to controls. GO and pathway analysis revealed that up-regulated mRNAs were involved in immune response, immune system process, system development or TNF signaling pathway, and antigen processing and presentation. To the contrary, down-regulated mRNAs participated in system development, regulation of biological processes and peroxisome proliferator-activated receptor (PPAR) signaling pathway. qRT-PCR results of 10 lncRNAs and mRNAs were consistent with the microarray data. M. tuberculosis infection of macrophages caused enhanced expression of lncRNA AK151345 in a time- and dose-dependent manner. We determined comprehensive expression profiles of differentially expressed lncRNAs in RAW264.7 macrophages infected by M. tuberculosis.

长的非编码RNA（lncRNA）已牵涉到生物过程的调控。lncRNA在巨噬细胞对结核分枝杆菌感染的应答中的作用尚未探索。我们使用高通量lncRNA基因芯片分析来检测是否存在结核分枝杆菌感染的RAW264.7巨噬细胞中差异表达的lncRNA和mRNA 。实时定量PCR（qRT-PCR）用于验证微阵列结果。使用生物信息学分析（GO和KEGG）来探索基因严重失调的功能。基因芯片结果表明，在患有结核分枝杆菌的RAW264.7巨噬细胞中差异表达了1,487个lncRNA（791个向上和696个向下）和910个mRNA（536个向上和374个向下）。与对照组相比 GO和途径分析表明，上调的mRNA与免疫反应，免疫系统过程，系统发育或TNF信号通路以及抗原加工和呈递有关。相反，下调的mRNA参与了系统开发，生物过程调节和过氧化物酶体增殖物激活受体（PPAR）信号通路。10个lncRNA和mRNA的qRT-PCR结果与微阵列数据一致。巨噬细胞的结核分枝杆菌感染以时间和剂量依赖性方式引起lncRNA AK151345的表达增强。我们确定了结核分枝杆菌感染的RAW264.7巨噬细胞中差异表达的lncRNAs的综合表达谱。