We have previously produced viral vectors (lentiviral vector, adenoviral vector, and adeno-associated viral vector) in small and in commercial scale in adherent cells using Pall fixed-bed iCELLis® bioreactor. Recently, a company called Univercells has launched a new fixed-bed bioreactor with the same cell growth surface matrix material, but with different fixed-bed structure than is used in iCELLis bioreactor. We sought to compare the new scale-X™ hydro bioreactor (2.4 m2) and iCELLis Nano system (2.67 m2) to see if the difference has any effect on cell growth or lentiviral vector and adenoviral vector productivity. Runs were performed using parameters optimized for viral vector production in iCELLis Nano bioreactor. Cell growth was monitored by counting nuclei, as well as by following glucose consumption and lactate production. In both bioreactor systems, cells grew well, and the cell distribution was found quite homogeneous in scale-X bioreactor. Univercells scale-X bioreactor was proven to be at least equally efficient or even improved in both lentiviral vector and adenoviral vector production. Based on the results, the same protocol and parameters used in viral vector production in iCELLis bioreactor can also be successfully used for the production in scale-X bioreactor system.

中文翻译：

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Scale-X 生物反应器系统在慢病毒和腺病毒载体生产中的基准测试。

我们之前已经使用颇尔固定床 iCELLis® 生物反应器在贴壁细胞中以小规模和商业规模生产病毒载体（慢病毒载体、腺病毒载体和腺相关病毒载体）。最近，一家名为 Univercells 的公司推出了一种新的固定床生物反应器，其细胞生长表面基质材料相同，但与 iCELLis 生物反应器中使用的固定床结构不同。我们试图比较新的 scale-X™ 水力生物反应器（2.4 平方米）和 iCELLis Nano 系统（2.67 平方米），看看差异是否对细胞生长或慢病毒载体和腺病毒载体的生产力有任何影响。使用针对 iCELLis Nano 生物反应器中病毒载体生产优化的参数进行运行。通过计数细胞核以及跟踪葡萄糖消耗和乳酸产生来监测细胞生长。在这两种生物反应器系统中，细胞生长良好，并且在 scale-X 生物反应器中发现细胞分布非常均匀。Univercells scale-X 生物反应器被证明在慢病毒载体和腺病毒载体生产方面至少具有同等效率，甚至有所改进。根据结果​​，在 iCELLis 生物反应器中用于病毒载体生产的相同协议和参数也可以成功用于 X 级生物反应器系统中的生产。