Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.

中文翻译：

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基于可追踪阻抗的活细胞分配和克隆。

单细胞克隆在干细胞生物学，癌症研究和生物技术中至关重要。监管机构现在需要无可争议的证明当前技术无法轻易提供的克隆性证明。在这里，我们报告使用工程移液器结合基于阻抗的传感头的一步克隆方法。这项技术可以对活细胞，干细胞和癌症干细胞进行有效且可追溯的分离，这些细胞可以在培养物中进行单独扩增并进行移植。