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Silencing circular RNA-ZNF652 represses proliferation and EMT process of renal carcinoma cells via raising miR-205.
Artificial Cells, Nanomedicine, and Biotechnology ( IF 5.8 ) Pub Date : 2020-02-20 , DOI: 10.1080/21691401.2020.1725532
Ludong Zhang 1 , Ya Guo 2
Affiliation  

Recently, the functions of circular RNAs (circRNAs) on cancer initiation and development arouse wide concern. Herein, we tested the influences of circ-ZNF652 on renal carcinoma cell growth and metastasis. Firstly, clinical renal carcinoma tissues and corresponding normal tissues were collected. The circ-ZNF652 expressions were tested. Then, the influences of silencing circ-ZNF652 on renal carcinoma A498 and ACHN cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) process, as well as Ras/Raf/MEK/ERK and JAK1/STAT3 pathways, were probed. Finally, whether miR-205 engaged in the influences of silencing circ-ZNF652 on A498 and ACHN cell were investigated. circ-ZNF652 had high expression level in clinical renal carcinoma tissues. Silencing circ-ZNF652 repressed A498 and ACHN cell proliferation and EMT process, but promoted cell apoptosis. Moreover, silencing circ-ZNF652 suppressed Ras/Raf/MEK/ERK and JAK1/STAT3 pathways in A498 and ACHN cells. Besides, the miR-205 expressions in A498 and ACHN cells were raised by silencing circ-ZNF652. Knockdown of miR-205 weakened the influences of silencing circ-ZNF652 on A498 and ACHN cell proliferation, apoptosis and EMT process. Silencing circ-ZNF652 repressed proliferation and EMT process of renal carcinoma A498 and ACHN cells via suppressing Ras/Raf/MEK/ERK and JAK1/STAT3 pathways, as well as raising miR-205 expression.

中文翻译:

沉默环状RNA-ZNF652通过提高miR-205抑制肾癌细胞的增殖和EMT过程。

最近,环状RNA(circRNA)在癌症的发生和发展中的功能引起了广泛的关注。在本文中,我们测试了circ-ZNF652对肾癌细胞生长和转移的影响。首先,收集临床肾癌组织和相应的正常组织。测试了circ-ZNF652表达。然后,探讨了沉默circ-ZNF652对肾癌A498和ACHN细胞增殖,凋亡和上皮-间质转化(EMT)过程以及Ras / Raf / MEK / ERK和JAK1 / STAT3途径的影响。最后,研究了miR-205是否参与了circ-ZNF652沉默对A498和ACHN细胞的影响。circ-ZNF652在临床肾癌组织中具有高表达水平。沉默circ-ZNF652抑制A498和ACHN细胞增殖和EMT过程,但促进细胞凋亡。此外,沉默circ-ZNF652抑制了A498和ACHN细胞中的Ras / Raf / MEK / ERK和JAK1 / STAT3途径。此外,沉默circ-ZNF652可提高A498和ACHN细胞中miR-205的表达。沉默miR-205可减弱circ-ZNF652沉默对A498和ACHN细胞增殖,凋亡和EMT过程的影响。沉默circ-ZNF652可以通过抑制Ras / Raf / MEK / ERK和JAK1 / STAT3途径以及提高miR-205的表达来抑制肾癌A498和ACHN细胞的增殖和EMT过程。沉默miR-205可减弱circ-ZNF652沉默对A498和ACHN细胞增殖,凋亡和EMT过程的影响。沉默circ-ZNF652抑制Ras / Raf / MEK / ERK和JAK1 / STAT3通路,并提高miR-205的表达,从而抑制了肾癌A498和ACHN细胞的增殖和EMT过程。抑制miR-205可减弱circ-ZNF652沉默对A498和ACHN细胞增殖,凋亡和EMT过程的影响。沉默circ-ZNF652可以通过抑制Ras / Raf / MEK / ERK和JAK1 / STAT3途径以及提高miR-205的表达来抑制肾癌A498和ACHN细胞的增殖和EMT过程。
更新日期:2020-12-01
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