In this study, the immobilization technology was used to improve the LA yield and shorten the fermentation time. The optimum conditions to immobilize Lactobacillus pentosus ATCC 8041 cell were determined by Taguchi design L16 (45). The immobilized L. pentosus ATCC 8041 cells prepared by 2% sodium alginate (SA) and 6% polyvinyl alcohol (PVA) with the immobilization process by 0.10 M calcium chloride (CaCl2) and 2.5% boric acid (H3BO3) had the best performance of LA yield at the temperature of 35 °C, which is significantly higher than that of L. pentosus ATCC 8041 free cells. These cells maintained the stable and efficient performance in 15 repeated batch fermentation, and they also have excellent mechanical strength to keep from breakage caused by cell growth and agitation.

中文翻译：

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优化戊糖乳杆菌细胞的固定条件。

在这项研究中，固定化技术被用来提高LA产量并缩短发酵时间。通过Taguchi设计L16（45）确定固定戊糖乳杆菌ATCC 8041细胞的最佳条件。由2％海藻酸钠（SA）和6％聚乙烯醇（PVA）制备的固定化戊糖乳杆菌ATCC 8041细胞在0.10 M氯化钙（CaCl2）和2.5％硼酸（H3BO3）的固定作用下具有最佳性能在35°C的温度下，LA的产量显着高于戊糖乳杆菌ATCC 8041游离细胞的产量。这些细胞在15次重复分批发酵中保持了稳定和高效的性能，并且它们还具有出色的机械强度，可防止细胞生长和搅拌引起的破损。