Yeasts are prominent hosts for the production of recombinant proteins from industrial enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways between these unicellular eukaryotic microorganisms and higher eukaryotic organisms has made them a preferential host to produce secretory recombinant proteins. However, there are several bottlenecks, in terms of quality and quantity, restricting their use as secretory recombinant protein production hosts. In this mini-review, we discuss recent developments in synthetic biology approaches to constructing yeast cell factories endowed with enhanced capacities of protein folding and secretion as well as designed targeted post-translational modification process functions. We focus on the new genetic tools for optimizing secretory protein expression, such as codon-optimized synthetic genes, combinatory synthetic signal peptides and copy number-controllable integration systems, and the advanced cellular engineering strategies, including endoplasmic reticulum and protein trafficking pathway engineering, synthetic glycosylation, and cell wall engineering, for improving the quality and yield of secretory recombinant proteins.

中文翻译：

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酵母合成生物学，用于设计生产分泌型重组蛋白的细胞工厂。

酵母是从工业酶到治疗蛋白生产重组蛋白的重要宿主。特别地，这些单细胞真核微生物与高级真核生物之间蛋白质分泌途径的相似性使其成为生产分泌性重组蛋白质的优先宿主。但是，就质量和数量而言，存在许多瓶颈，限制了它们作为分泌型重组蛋白生产宿主的用途。在这份小型综述中，我们讨论了合成生物学方法在构建酵母细胞工厂方面的最新进展，这些工厂具有增强的蛋白质折叠和分泌能力以及设计的靶向翻译后修饰过程功能。我们专注于优化分泌蛋白表达的新遗传工具，