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Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda.
International Journal of Genomics ( IF 2.9 ) Pub Date : 2020-01-04 , DOI: 10.1155/2020/5735968
Jiajia Wang 1, 2 , Jitao Li 1, 2 , Qianqian Ge 1, 2 , Zhao Chen 1, 2 , Jian Li 1, 2
Affiliation  

The Exopalaemon carinicauda could be a useful crustacean laboratory animal in many research fields. We newly established an inbred line of Exopalaemon carinicauda named EC4 inbred line by brother×sister mating and keeping to 11 generation. Trends in heterozygosity in the process of producing EC4 inbred line were examined through the characterization of polymorphisms based on gene frequencies of SNP and EST-SSR loci. The results demonstrated that the number of alleles (), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) gradually decreased with the increase of inbreeding generations. The genetic detection results indicated that 9 (29.03%, 9/31) of the SNP loci and 15 (32.61%, 15/46) of the EST-SSR loci were homozygous in 11 generation of EC4 inbred line. The variation of the growth-related traits, the immune responses, and antioxidant status were described in experimental full-sibling inbred populations of E. carinicauda at five levels of inbreeding coefficient (, , , , ) under controlled laboratory conditions. The body weight, body length, and survival rate in EC4 inbred line of all generations were less than the control population. Inbreeding affected the antibacterial activity, phenoloxidase (PO) activity, and superoxide dismutase (SOD) which decreased at the eleventh generation of EC4 inbred line. This study demonstrated that inbreeding had a negative effect on the economic traits and immune response, but our inbred line was established successfully until 11 and confirmed by genetic detection using SNP and EST-SSR loci.

中文翻译:

近交对Exopalaemon carinicauda的一个新近交系的遗传特性,生长,成活率和免疫反应的影响。

脊尾白虾可以在许多研究领域一个有用的甲壳类动物实验动物。我们通过兄弟×姐妹交配并维持到11代的新建立了Exopalaemon carinicauda的自交系,命名为EC4自交系。通过基于SNP和EST-SSR基因座的基因频率的多态性表征,研究了在生产EC4自交系的过程中杂合性的趋势。结果表明,等位基因数(),观察到的杂合度(Ho),预期的杂合度(He),并且随着近交世代的增加,多态信息含量(PIC)逐渐降低。遗传检测结果表明,在11代EC4近交系中,SNP基因座中有9个(29.03%,9/31)和EST-SSR基因座中有15个(32.61%,15/46)是纯合的。在五个近交系数水平上,在实验性全兄弟近亲E. carinicauda近交种群中描述了生长相关性状,免疫反应和抗氧化剂状态的变化( 在受控实验室条件下。所有世代EC4近交系的体重,体长和存活率均低于对照组。近亲繁殖影响了抗菌活性,酚氧化酶(PO)活性和超氧化物歧化酶(SOD),它们在EC4自交系的第11代时降低。这项研究表明,近交对经济性状和免疫应答具有负面影响,但我们的近交系已成功建立到11日,并通过SNP和EST-SSR基因座的基因检测得到了证实。
更新日期:2020-01-04
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