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Cryptosporidium and Giardia in locally harvested clams in Iqaluit, Nunavut.
Zoonoses and Public Health ( IF 2.4 ) Pub Date : 2020-02-17 , DOI: 10.1111/zph.12693
Anna J W Manore 1 , Sherilee L Harper 1, 2 , Jan M Sargeant 1, 3 , J Scott Weese 3 , Ashlee Cunsolo 4 , Anna Bunce 1 , Jamal Shirley 5 , Enooyaq Sudlovenick 6 , Karen Shapiro 1, 7
Affiliation  

High prevalences of Cryptosporidium and Giardia were recently found in enteric illness patients in the Qikiqtaaluk region of Nunavut, Canada, with a foodborne, waterborne or animal source of parasites suspected. Clams (Mya truncata) are a commonly consumed, culturally important and nutritious country food in Iqaluit; however, shellfish may concentrate protozoan pathogens from contaminated waters. The goal of this work was to investigate clams as a potential source of Cryptosporidium and Giardia infections in residents in Iqaluit, Nunavut. The objectives were to estimate the prevalence and genetically characterize Cryptosporidium and Giardia in locally harvested clams. Clams (n = 404) were collected from Iqaluit harvesters in September 2016. Haemolymph (n = 328) and digestive gland (n = 390) samples were screened for Cryptosporidium and Giardia via PCR, and amplified products were further processed for sequence analyses for definitive confirmation. Giardia DNA was found in haemolymph from 2 clams, while Cryptosporidium was not detected. The two Giardia sequences were identified as zoonotic Giardia enterica assemblage B. The overall prevalence of Giardia in clams near Iqaluit was low (0.6%) compared with other studies in southern Canada and elsewhere. The presence of Giardia DNA in clams suggests human or animal faecal contamination of coastal habitat around Iqaluit in shellfish harvesting waters. Results from this study are intended to inform public health practice and planning in Inuit Nunangat.

中文翻译:

努纳武特州伊卡卢伊特当地收获的蛤lam中的隐孢子虫和贾第虫。

最近在加拿大努纳武特州的奇基夸塔鲁克地区的肠道疾病患者中发现了隐孢子虫和贾第鞭毛虫的高流行,疑似有食源性,水源性或动物性寄生虫。蛤((Mya truncata)是伊卡卢特(Iqaluit)常见的具有文化重要性和营养的乡村食品。但是,贝类可能会浓缩受污染水域中的原生动物病原体。这项工作的目的是调查蛤N作为努纳武特州伊卡卢伊特居民隐孢子虫和贾第鞭毛虫感染的潜在来源。目的是估计当地收获的蛤中的隐孢子虫和贾第鞭毛虫的患病率,并对它们进行遗传鉴定。2016年9月从伊卡卢伊特(Iqaluit)收割机采集了蛤(n = 404)。通过PCR筛选了血淋巴(n = 328)和消化腺(n = 390)样品中的隐孢子虫和贾第鞭毛虫,并进一步处理了扩增产物以进行序列分析以进行最终确认。在2只蛤的血淋巴中发现了贾第鞭毛虫DNA,而未检测到隐孢子虫。这两个贾第虫序列被鉴定为人畜共患的贾第虫肠杆菌组合B。与加拿大南部和其他地区的其他研究相比,贾第鞭毛虫在伊卡卢伊特附近蛤lam中的总体患病率较低(0.6%)。蛤中贾第鞭毛虫DNA的存在表明人类或动物粪便污染了贝类捕捞水中伊卡卢伊特周围的沿海生境。这项研究的结果旨在为因纽特人努南加特的公共卫生实践和规划提供参考。然后对扩增产物进行进一步处理,以进行序列分析以进行最终确认。在2只蛤的血淋巴中发现了贾第鞭毛虫DNA,而未检测到隐孢子虫。这两个贾第虫序列被鉴定为人畜共患的贾第虫肠杆菌组合B。与加拿大南部和其他地区的其他研究相比,贾第鞭毛虫在伊卡卢伊特附近蛤lam中的总体患病率较低(0.6%)。蛤中贾第鞭毛虫DNA的存在表明人类或动物粪便污染了贝类捕捞水中伊卡卢伊特周围的沿海生境。这项研究的结果旨在为因纽特人努南加特的公共卫生实践和规划提供参考。然后对扩增产物进行进一步处理,以进行序列分析以进行最终确认。在2只蛤的血淋巴中发现了贾第鞭毛虫DNA,而未检测到隐孢子虫。这两个贾第虫序列被鉴定为人畜共患的贾第虫肠杆菌组合B。与加拿大南部和其他地区的其他研究相比,贾第鞭毛虫在伊卡卢伊特附近蛤c中的总体患病率较低(0.6%)。蛤中贾第鞭毛虫DNA的存在表明人类或动物粪便污染了贝类捕捞水中伊卡卢伊特周围的沿海生境。这项研究的结果旨在为因纽特人努南加特的公共卫生实践和规划提供参考。与加拿大南部和其他地区的其他研究相比,伊卡鲁伊特附近蛤lam的贾第鞭毛虫总体患病率较低(0.6%)。蛤中贾第鞭毛虫DNA的存在表明人类或动物粪便污染了贝类捕捞水中伊卡卢伊特周围的沿海生境。这项研究的结果旨在为因纽特人努南加特的公共卫生实践和规划提供参考。与加拿大南部和其他地区的其他研究相比,伊卡鲁伊特附近蛤lam的贾第鞭毛虫总体患病率较低(0.6%)。蛤中贾第鞭毛虫DNA的存在表明人类或动物粪便污染了贝类捕捞水中伊卡卢伊特周围的沿海生境。这项研究的结果旨在为因纽特人努南加特的公共卫生实践和规划提供参考。
更新日期:2020-02-17
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