Tropical race 4 of Fusarium oxysporum f. sp. cubense (FocTR4) is seriously threatening the banana industry worldwide. Resistant genotypes are present in wild relatives of banana, but little is known about the genetic and molecular mechanisms driving resistance responses. In this work, through in-depth expression analysis, we compared the responses of the resistant wild relative Musa acuminata ssp. burmanicoides (WTB) with the susceptible banana cultivar “Brizilian” (CAV, as it belongs to the Cavendish subgroup) to FocTR4 infection. Our findings showed that 1196 defense-related genes in the resistant WTB were differentially expressed genes (DEGs); only 358 defense-related DEGs were detected in CAV. DEGs related to pattern recognition receptors (PRRs) and disease resistance (R genes) were found in both genotypes, indicating the onset of both basal and specific defenses to FocTR4. Genes associated with cell wall modification exhibited a more remarkable upregulation in WTB than in CAV and might be involved in resistance during penetration steps. Our data also suggested that the high resistance of WTB is quantitatively driven with larger numbers and higher expression levels of defense-related DEGs. Fine-tuning studies to understand the resistance responses of WTB at early stages should be conducted to better support banana breeding programs. Further investigations are also required to validate the role of key genes screened in this study.

中文翻译：

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深入的RNA序列分析揭示了野生香蕉对尖孢镰刀菌f的相对抗性的关键响应方面。sp。立方热带种族4。

尖孢镰刀菌f的热带种族4 。sp。cubense（FocTR4）严重威胁着全球范围内的香蕉产业。抗性基因型存在于香蕉的野生近缘种中，但对驱动抗性反应的遗传和分子机制知之甚少。在这项工作中，通过深入的表达分析，我们比较了抗药性野生亲缘对接草（Musa acuminata ssp）的反应。burmanicoides（WTB）感染FocTR4的香蕉易感品种“ Brizilian”（CAV，属于卡文迪许亚组）。我们的研究结果表明，抗性WTB中的1196个与防御相关的基因是差异表达基因（DEGs）。在CAV中仅检测到358个与国防相关的DEG。与模式识别受体（PRR）和抗病性有关的DEG（R两种基因型中都发现了这些基因），这表明FocTR4的基础防御和特异性防御​​都开始了。与细胞壁修饰相关的基因在WTB中比在CAV中表现出更显着的上调，并且可能在穿透步骤中参与抗性。我们的数据还表明，WTB的高抗性在数量上和与防御相关的DEG的较高表达水平之间存在定量关系。为了更好地支持香蕉育种计划，应该进行微调研究以了解WTB在早期的抗药性。还需要进一步的研究以验证在这项研究中筛选的关键基因的作用。