Abstract

The aim of the present study was to estimate the potential toxic effects of a subacute and a subchronic exposure to methomyl (MET) on some parts of the digestive system in male albino rats. Wistar rats were exposed daily by oral gavage to 4 mg/kg body weight (b.w.) of MET over 28 days in subacute experiments and to 1 mg/kg b.w. of MET for 90 consecutive days in subchronic exposure. Plasma levels of liver function indicators (ALT, AST, ALP and LDH) were significantly elevated after administration of MET to rats. MET had significant pro-oxidative effects on the intestines, as indicated by decreases in nonenzymatic (GSH) and enzymatic antioxidant (GPx, GST and CAT) parameters and significant increases in TBARS levels in both the duodenum and colon of rats. Microscopically, the duodenum and colon of rats exposed to MET showed severe pathological changes. It could be concluded that MET profoundly altered the structure and antioxidant status of intestinal rat tissue, which might lead to intestinal and digestive dysfunctions.

摘要

本研究的目的是评估亚急性和亚慢性暴露于灭多威 (MET) 对雄性白化大鼠消化系统某些部位的潜在毒性影响。在亚急性实验中，Wistar 大鼠在 28 天内每天通过口服强饲法暴露于 4 毫克/千克体重 (bw) 的 MET，在亚慢性暴露中连续 90 天暴露于 1 毫克/千克体重的 MET。大鼠给予 MET 后，血浆肝功能指标（ALT、AST、ALP 和 LDH）水平显着升高。MET 对肠道具有显着的促氧化作用，表现为大鼠十二指肠和结肠中非酶 (GSH) 和酶抗氧化剂 (GPx、GST 和 CAT) 参数的降低以及 TBARS 水平的显着增加。显微镜下，暴露于MET的大鼠十二指肠和结肠出现严重的病理变化。可以得出结论，MET深刻改变了大鼠肠道组织的结构和抗氧化状态，可能导致肠道和消化功能障碍。