Although reactive oxygen species in semen are associated with unfavorable results with respect to assisted reproductive technology, their effects based on the detailed stages of embryo development are unclear. We investigated the relationship between reactive oxygen species in semen and the oocyte fertilization rate, cleavage rate, and blastulation rate of intracytoplasmic sperm injections. This retrospective study enrolled 77 couples who underwent intracytoplasmic sperm injection and analyzed 887 eggs from 141 cycles of intracytoplasmic sperm injection. The reactive oxygen species level in semen was compared between the fertilized and nonfertilized groups, between the good-cleavage-embryo and non-developed-embryo groups, and between the good-quality-blastocyst and poor-quality-blastocyst groups. The cut-off level of reactive oxygen species was calculated to predict good-cleavage-embryo and good-quality-blastocyst development. The fertilization rate was 65.4%, and the mean reactive oxygen species levels were not significantly different between the fertilized and nonfertilized groups. The reactive oxygen species level was significantly higher in the non-developed-embryo group than in the good-cleavage-embryo group (P = 0.026) and was significantly lower in the good-quality-blastocyst group than in the poor-quality-embryo group (P = 0.015). Cleavage embryos and blastocysts were divided into high- and low-reactive-oxygen-species groups using a cut-off value of 6601 and 4926 relative light units, as calculated from the receiver operating characteristic curve. The rates of good-cleavage embryos and good-quality blastocysts were lower in the high-reactive-oxygen-species group than in the low-reactive-oxygen-species group, which were both statistically significant. To conclude, reactive oxygen species in semen is considered to have an adverse effect on both the early and late stages of embryo development in intracytoplasmic sperm injection.Abbreviations: GnRH, gonadotropin-releasing hormone; ICSI, intracytoplasmic sperm injection; IVF, in vitro fertilization; LPO, lipid peroxidation; NADPH, nicotinamide adenine dinucleotide phosphate; RLU, relative light units; ROC, receiver operating characteristic; ROS, reactive oxygen species.

中文翻译：

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精液中活性氧的早期和晚期父体作用对胞浆内精子注射后胚胎发育的影响。

尽管精液中的活性氧与辅助生殖技术的不良结果有关，但基于胚胎发育的详细阶段，它们的作用尚不清楚。我们研究了精液中活性氧与卵母细胞内精子注射卵母细胞受精率，卵裂率和成胚率之间的关系。这项回顾性研究招募了77对进行了胞浆内精子注射的夫妇，并分析了141个胞浆内精子注射周期中的887个卵。比较了受精组和未受精组，卵裂良好和未发育胚组以及优质胚泡和不良胚泡组精液中的活性氧水平。计算出活性氧种类的截止水平，以预测良好的卵裂胚和优质的胚泡发育。受精率为65.4％，受精组和未受精组之间的平均活性氧水平没有显着差异。非发育胚胎组的活性氧水平显着高于卵裂良好的组（P = 0.026），优质胚泡组的活性氧水平显着低于不良卵胚组组（P = 0.015）。根据接受者的工作特征曲线计算，使用6601和4926相对光单位的临界值，将卵裂的胚胎和胚泡分为高反应性和低反应性氧种类。高活性氧种组的卵裂好率和优质囊胚的发生率均低于低活性氧种组，两者均具有统计学意义。总之，精液中的活性氧被认为对细胞质内精子注射的胚胎发育的早期和晚期都有不利影响。缩写：GnRH，促性腺激素释放激素；ICSI，胞浆内精子注射；IVF，体外受精；LPO，脂质过氧化；NADPH，烟酰胺腺嘌呤二核苷酸磷酸；RLU，相对光单位；ROC，接收机工作特性；ROS，活性氧。精液中的活性氧被认为对细胞质内精子注射的胚胎发育的早期和晚期都有不利影响。缩写：GnRH，促性腺激素释放激素；ICSI，胞浆内精子注射；IVF，体外受精；LPO，脂质过氧化；NADPH，烟酰胺腺嘌呤二核苷酸磷酸；RLU，相对光单位；ROC，接收机工作特性；ROS，活性氧。精液中的活性氧被认为对细胞质内精子注射的胚胎发育的早期和晚期都有不利影响。缩写：GnRH，促性腺激素释放激素；ICSI，胞浆内精子注射；IVF，体外受精；LPO，脂质过氧化；NADPH，烟酰胺腺嘌呤二核苷酸磷酸；RLU，相对光单位；ROC，接收机工作特性；ROS，活性氧。