Females of the Queensland fruit fly (QFF), Bactrocera tryoni, are amongst the most damaging pests of horticulture in Australia and neighboring countries. Females can lay eggs into more than a hundred species of fruits and vegetables, resulting in large crop losses. Sexually mature males can be managed sustainably with traps baited with long-lasting synthetic lures, and sexually immature males and females can be attracted and killed by short-lived protein baits applied directly on surfaces, with a low success rate (< 20%). No long-lasting attractants for virgin or mated females exist. With the aim of developing a female attractant for surveillance, we collected and analyzed the odors of four ripe host fruits: orange, cherry guava, banana and feijoa. Virgin and mated female QFF were tested with gas-chromatography coupled with electro-antennographic detection to identify electrophysiologically (EAD)-active compounds. We detected 41 EAD-active compounds, with seven found common for more than one fruit. Overall, mated females responded more often and with higher intensity than virgin females. In particular, five compounds present either in cherry guava or feijoa triggered a significantly higher EAD response from mated females than from virgins. Twenty-six EAD-active compounds were selected and tested individually in a Y-tube olfactometer to measure attraction of both virgin and mated females. Behavioral responses differed significantly amongst the compounds, but not strongly between virgin and mated females. We did not find any correlation between electrophysiological and behavioral responses. Further field testing with behaviorally-active compounds is needed for the development of a new QFF female lure.

中文翻译：

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昆士兰果蝇雌对果味的电生理和行为响应。

昆士兰果蝇（QFF）的女性，昆士兰实蝇是澳大利亚和邻国破坏性最大的园艺害虫之一。雌性可以将卵产于一百多种水果和蔬菜中，从而导致大量农作物损失。对于性成熟的雄性，可以使用诱饵持久诱饵诱捕，以可持续的方式进行管理；对于性未成熟的雄性和雌性，可以通过直接在表面上施用的短寿命蛋白诱饵来吸引和杀死，成功率较低（<20％）。不存在用于处女或交配雌性的长效引诱剂。为了开发一种用于监视的女性引诱剂，我们收集并分析了四种成熟寄主果实的气味：橙，樱桃番石榴，香蕉和美jo。使用气相色谱法和电造影检查法对初生和交配雌性QFF进行测试，以鉴定具有电生理（EAD）活性的化合物。我们检测到41种EAD活性化合物，其中7种是一种以上水果常见的。总体而言，与未婚女性相比，交配女性的反应频率更高，强度更高。特别是，樱桃番石榴或美酒中存在的五种化合物引发的交配雌性的EAD响应明显高于处女。选择了26种EAD活性化合物，并分别在Y型管嗅觉仪中进行了测试，以测量原始女性和交配女性的吸引力。化合物之间的行为反应差异显着，但未婚女性和交配女性之间的行为反应差异不大。我们没有发现电生理反应和行为反应之间的任何关联。