A simple, sensitive, low-cost, quick and reliable square-wave anodic stripping voltammetric method is described for the determination of the antidiabetic drug Linagliptin (LNG) in pure form, tablets, and spiked human urine and plasma samples. Using a pencil graphite electrode (PGE), cyclic voltammetry (CV) was applied to study the electrochemical behavior of LNG. In a Teorell–Stenhagen buffer (pH 5.5) containing 0.1 M NaClO₄ as a supporting electrolyte, the LNG yields an irreversible well-defined oxidation peak at about 1.2 V vs. Ag/AgCl electrode. The various affecting factors, such as the pH, buffer type, supporting electrolyte, accumulation potential, scan rate and accumulation time, were tested and optimized. Also, square-wave adsorptive anodic stripping voltammetric (SWAdASV) studies show that the peak current various linearly over the LNG concentration range of 0.24 – 5.20 μg mL⁻¹ (R² = 0.9994). The detection and quantification limits were calculated to be 0.10 and 0.33 μg mL⁻¹, respectively. The proposed procedure exhibits a good precision, selectivity, and stability and was applied successfully to determine the LNG in pharmaceutical formulations (tablets) and biological fluids (spiked human urine and plasma samples).

描述了一种简单、灵敏、低成本、快速且可靠的方波阳极溶出伏安法，用于测定纯品、片剂以及加标的人尿和血浆样品中的抗糖尿病药物利格列汀 (LNG)。使用铅笔石墨电极（PGE），应用循环伏安法（CV）研究液化天然气的电化学行为。在含有 0.1 M NaClO ₄作为支持电解质的 Teorell-Stenhagen 缓冲液 (pH 5.5) 中，LNG 在约 1.2 V（相对于Ag/AgCl 电极）处产生不可逆的明确氧化峰。对pH、缓冲液类型、支持电解质、富集电位、扫描速率和富集时间等各种影响因素进行了测试和优化。^{此外，方波吸附阳极溶出伏安法 (SWAdASV) 研究表明，峰值电流在 0.24 – 5.20 µg mL -1} ( R ² = 0.9994)的 LNG 浓度范围内呈线性变化。计算出检测限和定量限分别为0.10和0.33 μg mL ^-1。所提出的程序具有良好的精度、选择性和稳定性，并成功应用于测定药物制剂（片剂）和生物液体（加标的人体尿液和血浆样品）中的 LNG。