In the present study, we investigated the expression of protein kinase C (PKC) signaling during the embryonic diapause process of Bombyx mori. PKC activity, determined using an antibody to phosphorylated substrates of PKC, was found to be significantly higher in developing eggs as compared to that of diapause eggs. In eggs whose diapause initiation was prevented by HCl, non-diapause eggs, and eggs in which diapause had been terminated by chilling of diapausing eggs at 5 °C for 70 days and then were transferred to 25 °C, PKC-dependent phosphorylation levels of multiple proteins showed dramatic stage-dependent increases compared to those of diapause eggs. Higher protein levels of PKC were also detected in developing eggs as compared to those of diapause eggs. Determination of PKC enzymatic activity during the middle embryonic stage showed higher PKC activity in developing eggs compared to diapause eggs, thus further confirming differential regulation of PKC signaling during the embryonic diapause process. Examination of temporal changes in mRNA expression levels of classical PKC (cPKC) and atypical PKC (aPKC) showed no difference between diapause and HCl-treated eggs. These results demonstrated that differential expressions of PKC signaling between diapause and developing eggs are related to the embryonic diapause process of B. mori.

中文翻译：

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蛋白激酶C的表达与家蚕胚胎滞育过程有关。

在本研究中，我们调查了家蚕胚胎滞育过程中蛋白激酶C（PKC）信号的表达。与滞育卵相比，在发育卵中发现使用PKC磷酸化底物抗体测定的PKC活性显着更高。在用HCl阻止了滞育的卵中，非滞育的卵和通过在5°C下将滞育的卵冷却70天而终止了滞育的卵，然后转移到25°C，PKC依赖的磷酸化水平为与滞育卵相比，多种蛋白表现出显着的阶段依赖性增加。与滞育卵相比，在发育中的卵中还检测到较高的PKC蛋白水平。与滞育卵相比，在中期胚胎阶段对PKC酶活性的测定显示较高的PKC活性，从而进一步证实了在胚胎滞育过程中PKC信号的差异调节。检查经典PKC（cPKC）和非典型PKC（aPKC）的mRNA表达水平的时间变化，表明滞育和盐酸处理的卵之间无差异。这些结果证明滞育和发育中的卵之间PKC信号转导的差异表达与桑蚕的胚胎滞育过程有关。检查经典PKC（cPKC）和非典型PKC（aPKC）的mRNA表达水平的时间变化，表明滞育和盐酸处理的卵之间无差异。这些结果证明滞育和发育中的卵之间PKC信号转导的差异表达与桑蚕的胚胎滞育过程有关。检查经典PKC（cPKC）和非典型PKC（aPKC）的mRNA表达水平的时间变化，表明滞育和盐酸处理的卵之间无差异。这些结果证明滞育和发育中的卵之间PKC信号转导的差异表达与桑蚕的胚胎滞育过程有关。