RATIONALE The offline coupling of high-performance thin-layer chromatography (HPTLC) with atmospheric solids analysis probe mass spectrometry (ASAP-MS) was evaluated for the characterization of polymeric additives in gasoline. METHODS A protocol was developed to optimize the ion signal. A glass capillary was moistened with deionized water, and then dipped into silica gel scratched from an HPTLC plate. The capillary tube was fixed to the ASAP holder and introduced into the ionization source for analysis by MS. Silica gel, reversed-phase C18 and cellulose stationary phases were evaluated. RESULTS The effect of the stationary phase and the nature of analyte were evaluated using polypropylene glycol and polyisobutylene succinimide polyamine as analyte molecules. The optimal ionization conditions are significantly different between ASAP and HPTLC/ASAP-MS analyses. In particular, a higher desorption gas temperature was required to produce ions from the silica gel HPTLC plate. The presence of the stationary phase reduces the internal energy of the ions and limits the fragmentation. CONCLUSIONS HPTLC/ASAP-MS is a very fast and efficient technique for the analysis of polymers in formulated fuels. Good ionization efficiency was obtained with all investigated stationary phases.

中文翻译：

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具有大气固体分析探针质谱的高性能薄层色谱，用于分析汽油聚合物添加剂。

理由评估了高性能薄层色谱法（HPTLC）与大气固体分析探针质谱法（ASAP-MS）的离线偶联，以表征汽油中的聚合物添加剂。方法制定了优化离子信号的方案。将玻璃毛细管用去离子水润湿，然后浸入从HPTLC板刮下的硅胶中。毛细管固定在ASAP固定器上，并引入电离源中，以进行MS分析。评估了硅胶，反相C18和纤维素固定相。结果使用聚丙二醇和聚异丁烯琥珀酰亚胺多胺作为分析物分子，评估了固定相的影响和分析物的性质。ASAP和HPTLC / ASAP-MS分析之间的最佳电离条件明显不同。特别地，需要更高的解吸气体温度以从硅胶HPTLC板产生离子。固定相的存在会减少离子的内能并限制碎片。结论HPTLC / ASAP-MS是一种非常快速高效的技术，可用于分析配制燃料中的聚合物。在所有研究的固定相中均获得了良好的电离效率。结论HPTLC / ASAP-MS是一种非常快速高效的技术，可用于分析配制燃料中的聚合物。在所有研究的固定相中均获得了良好的电离效率。结论HPTLC / ASAP-MS是一种非常快速高效的技术，可用于分析配制燃料中的聚合物。在所有研究的固定相中均获得了良好的电离效率。