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Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration.
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-02-14 , DOI: 10.1007/s10482-020-01393-7
Karina Ramijan 1 , Zheren Zhang 1 , Gilles P van Wezel 1 , Dennis Claessen 1
Affiliation  

Filamentous Actinobacteria are multicellular bacteria with linear replicons. Kitasatospora viridifaciens DSM 40239 contains a linear 7.8 Mb chromosome and an autonomously replicating plasmid KVP1 of 1.7 Mb. Here we show that lysozyme-induced protoplast formation of the multinucleated mycelium of K. viridifaciens drives morphological diversity. Characterisation and sequencing of an individual revertant colony that had lost the ability to differentiate revealed that the strain had not only lost most of KVP1 but also carried deletions in the right arm of the chromosome. Strikingly, the deletion sites were preceded by insertion sequence elements, suggesting that the rearrangements may have been caused by replicative transposition and homologous recombination between both replicons. These data indicate that protoplast formation is a stressful process that can lead to profound genetic changes.

中文翻译:

丝状细菌Kitasatospora viridifaciens中的基因组重排和大质粒损失与原生质体的形成和再生有关。

丝状放线菌是具有线性复制子的多细胞细菌。Kitaritospora viridifaciens DSM 40239包含一个7.8 Mb线性染色体和一个1.7 Mb自主复制质粒KVP1。在这里,我们显示溶菌酶诱导的K. viridifaciens多核菌丝体的原生质体形成驱动了形态多样性。丧失了分化能力的单个逆转录菌落的鉴定和测序表明,该菌株不仅丧失了大部分KVP1,而且在染色体的右臂也进行了缺失。令人惊讶的是,缺失位点之前是插入序列元件,这表明重排可能是由两个复制子之间的复制性转座和同源重组引起的。
更新日期:2020-02-14
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