Tear cytokine levels in the diagnosis and severity assessment of ocular chronic graft-versus-host disease(GVHD).,The Ocular Surface

当前位置： X-MOL 学术 › Ocul. Surf. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Tear cytokine levels in the diagnosis and severity assessment of ocular chronic graft-versus-host disease(GVHD).
The Ocular Surface ( IF 6.4 ) Pub Date : 2020-01-15 , DOI: 10.1016/j.jtos.2019.12.005
Bohao Hu ₁ , Yuan Qiu ₁ , Jing Hong ₁

Affiliation

Purpose

To analyse the tear cytokine levels of patients diagnosed with ocular chronic graft-versus-host disease(GVHD)and examine the consistency of these levels with different ocular surface parameters.

Methods

23 ocular chronic GVHD patients were selected for the assessment of tear cytokine levels and ocular surface parameters (TBUT, CFS, OSDI, and Schirmer's test), and 16 dry eye disease(DED)patients without systemic immune disease were selected as the control group. The 23 cytokines were measured using microsphere-based immunoassay analysis and their consistency with different ocular surface parameters was studied.

Results

ICAM-1, IL-1β, IL-6, and IL-8 showed elevated levels in the eyes of oGVHD patients compared to DED [P < 0.001]. IL-7 and EGF showed lower levels in the eyes of patients with oGVHD than in those of patients with DED [P < 0.0001]. Furthermore, the levels of IL-6 and IL-8 showed a stronger correlation with corneal fluorescein staining (CFS) (P < 0.05), and the levels of IL-6 and ICAM-1 were most consistent with fluorescein tear film break-up time (TBUT) (P < 0.05).

Conclusions

Our study demonstrated certain tear cytokines, including ICAM-1, IL-1β, IL-6, IL-8 IL-7 and EGF, as promising new possible diagnostic markers of chronic oGVHD and criteria for chronic ocular GVHD severity. Because tear sampling is noninvasive and simple, this method is expected to be an overwhelming applicable for the screening and diagnosis of chronic GVHD.

中文翻译：

眼细胞因子水平在眼慢性移植物抗宿主病（GVHD）的诊断和严重程度评估中。

目的

分析被诊断为眼部慢性移植物抗宿主病（GVHD）的患者的泪液细胞因子水平，并检查这些水平与不同眼表参数的一致性。

方法

选择23例慢性GVHD眼患者评估泪液细胞因子水平和眼表参数（TBUT，CFS，OSDI和Schirmer's test），并选择16例无全身免疫性疾病的干眼病（DED）患者作为对照组。使用基于微球的免疫测定分析测量了23种细胞因子，并研究了它们与不同眼表参数的一致性。

结果

与DED相比，oGVHD患者的眼中ICAM-1，IL-1β，IL-6和IL-8的水平升高[P <0.001]。oGVHD患者的眼中IL-7和EGF的水平低于DED患者的眼中的水平[P <0.0001]。此外，IL-6和IL-8的水平与角膜荧光素染色（CFS）有更强的相关性（P <0.05），IL-6和ICAM-1的水平与荧光素泪膜破裂最一致。时间（TBUT）（P <0.05）。