The study aims to explore the combinatorial effect of naringin with antibiotics, ciprofloxacin and tetracycline on Pseudomonas aeruginosa biofilms. The antibiofilm efficacy of selected treatment regimes against P. aeruginosa biofilm were quantified by crystal violet assay, MTT assay, Congo red binding assay, and were visualized by confocal laser scanning microscopy and scanning electron microscopy. All the assays reflected antibiofilm activities, however, combinatorial performances of naringin with antibiotics were found to be more significant. A significant reduction in swimming and swarming motilities along with pellicle formation and altered colony morphology were observed as a result of combinatorial effect. The cytotoxicity of naringin and its antibiotic combinations was assayed on murine macrophage cell line. The applicability of such combinations was tested for their relative eradication against pre-formed biofilm on urinary catheter surface. This finding indicated that naringin potentiates the efficacy of both ciprofloxacin and tetracycline on P. aeruginosa biofilm in comparison to their solo treatment. The finding would help to open hitherto unexplored possibilities of establishing naringin as a potential antibiofilm agent and suggest on the possibility of its use in drug-herb combinations for managing biofilm-associated bacterial infections.

该研究旨在探讨柚皮苷与抗生素，环丙沙星和四环素对铜绿假单胞菌生物膜的联合作用。所选治疗方案对铜绿假单胞菌的抗生物膜功效通过结晶紫测定，MTT测定，刚果红结合测定对生物膜进行定量，并通过共聚焦激光扫描显微镜和扫描电子显微镜对生物膜进行可视化。所有的测定都反映了抗生物膜的活性，但是，柚皮苷与抗生素的组合性能更显着。由于组合效应，观察到游泳和成群的移动性显着降低，并有表膜形成和菌落形态改变。在鼠巨噬细胞系上测定柚皮苷及其抗生素组合的细胞毒性。测试了这些组合的适用性，以相对根除在导尿管表面上预先形成的生物膜。这一发现表明，柚皮苷增强了环丙沙星和四环素的疗效。铜绿假单胞菌生物膜与其单独处理相比。该发现将有助于打开迄今尚未开发的将柚皮苷确立为潜在抗生物膜剂的可能性，并建议将其用于药物-草药组合中以管理与生物膜相关的细菌感染。