Virus contamination events in cell culture-based biotechnology processes have occurred and have had a dramatic impact on the supply of life-saving drugs, and thus on the wellbeing of patients. Cleanup requires effective and robust virucidal decontamination procedures for both the liquid reactor content before discharge, as well as facility surfaces to prevent recurrence. Beyond rare contamination events, it is important to implement virucidal disinfection for change-over procedures as effective preventive measure in routine biomanufacturing. Knowledge of the virus inactivation capacity of commonly used disinfectants is therefore important. However, available virus inactivation data often refer to studies performed in suspension only, and not, as often more relevant, to virus inactivation on surfaces. In this study three liquid disinfectants, based on sodium hypochlorite, glutaraldehyde, or hydrogen peroxide/peroxyacetic acid, as well as one gaseous hydrogen peroxide-based disinfectant were investigated for inactivation of lipid enveloped and non-lipid enveloped model viruses, using suspension (for the liquid disinfectants) and carrier assay designs for their virucidal efficacy on surface. The results of these side-by-side investigations demonstrate that depending on the type of application, i.e. routine surface disinfection or decontamination of e.g. a contaminated bioreactor content, the most effective choice of disinfectant may be remarkably different.

基于细胞培养的生物技术过程中的病毒污染事件已经发生，并且对挽救生命的药物的供应，从而对患者的健康产生了巨大的影响。清理需要对排放前的液体反应器内容物以及设施表面进行有效而稳健的杀菌消毒程序，以防止再次发生。除了罕见的污染事件外，对于常规的生物生产过程，对转换程序实施杀毒消毒作为有效的预防措施也很重要。因此，了解常用消毒剂的病毒灭活能力很重要。但是，可用的病毒灭活数据通常是指仅在悬浮液中进行的研究，而通常与表面病毒灭活没有太大关系。在这项研究中，三种液体消毒剂 使用悬浮液（用于液体消毒剂）和载体测定法研究了基于次氯酸钠，戊二醛或过氧化氢/过氧乙酸以及一种基于气态过氧化氢的消毒剂对脂质被膜和非脂质被膜模型病毒的灭活作用设计其表面杀虫功效。这些并行研究的结果表明，根据应用类型（即常规的表面消毒或对受污染的生物反应器内容物的净化），消毒剂的最有效选择可能会明显不同。使用悬浮液（用于液体消毒剂）和载体测定设计，以了解其在表面上的杀虫功效。这些并行研究的结果表明，根据应用类型（即常规的表面消毒或对受污染的生物反应器内容物的净化），消毒剂的最有效选择可能会明显不同。使用悬浮液（用于液体消毒剂）和载体测定设计，以了解其在表面上的杀菌功效。这些并行研究的结果表明，根据应用类型（即常规的表面消毒或对受污染的生物反应器内容物的净化），消毒剂的最有效选择可能会明显不同。