Two Pythium‐infested soils were used to compare the wheat root and rhizosphere soil microbial communities from plants grown in the field or in greenhouse trials and their stability in the presence of biocontrol agents. Bacteria showed the highest diversity at early stages of wheat growth in both field and greenhouse trials, while fungal diversity increased later on, at 12 weeks of the crop cycle. The microbial communities were stable in roots and rhizosphere samples across both soil types used in this study. Such stability was also observed irrespective of the cultivation system (field or greenhouse) or addition of biocontrol coatings to wheat seeds to control Pythium disease (in this study soil infected with Pythium sp. clade F was tested). In greenhouse plant roots, Archaeorhizomyces, Debaryomyces, Delftia, and unclassified Pseudeurotiaceae were significantly reduced when compared to plant roots obtained from the field trials. Some operational taxonomic units (OTUs) represented genetic determinants clearly transmitted vertically by seed endophytes (specific OTUs were found in plant roots) and the plant microbiota was enriched over time by OTUs from the rhizosphere soil. This study provided key information regarding the microbial communities associated with wheat roots and rhizosphere soils at different stages of plant growth and the role that Paenibacillus and Streptomyces strains play as biocontrol agents in supporting plant growth in infested soils.

中文翻译：

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在存在生物控制剂佩氏芽孢杆菌SP9和富链链霉菌FU14的田间和温室试验中，类似的小麦根际根际微生物演替。

使用两种腐霉菌感染的土壤来比较田间或温室试验中生长的植物的小麦根和根际土壤微生物群落及其在生物防治剂存在下的稳定性。在田间试验和温室试验中，细菌在小麦生长的早期都表现出最高的多样性，而在作物周期的12周之后，真菌的多样性则增加了。在本研究中使用的两种土壤类型中，根和根际样品中的微生物群落均稳定。无论采用何种耕作系统（田间或温室）或向小麦种子中添加生物防治涂层来控制腐霉病（在本研究中感染了腐霉菌的土壤），均观察到了这种稳定性。sp。进化枝F已经过测试）。在温室植物的根，Archaeorhizomyces，德巴利酵母属，代尔夫特，和未分类Pseudeurotiaceae进行显著与从现场试验得到的植物的根时减小。一些操作性分类单位（OTU）代表了遗传决定因素，这些决定因素是种子内生菌清楚地垂直传播的（在植物根系中发现了特定的OTU），并且随着时间的推移，根际土壤中的OTU丰富了植物微生物群。这项研究提供了有关植物生长不同阶段与小麦根和根际土壤相关的微生物群落的重要信息，以及Paenibacillus和Streptomyces的作用 菌株在支持受侵染土壤中植物生长方面起着生防作用。