Renalase is predominantly expressed in the kidney, where it plays a role in catecholamine metabolism and blood pressure regulation. Moderate‐intensity exercise (MEX) has been shown to increase the concentration of renalase in the blood and to reduce renal function in humans. Moreover, such exercise was also reported to increase catecholamine levels. Here, we examined renalase concentration in the blood and renalase expression levels in different organs after MEX in rats. Twelve male Wistar rats were made to run on a treadmill (MEX group) for 60 min at 20 m·min⁻¹, after resting for 15 min. The control group rats were euthanized after resting on the treadmill. Tissue and blood samples were analyzed using western blotting, real‐time RT‐PCR and ELISA. Overall, the concentrations of renalase in the blood were significantly higher in the MEX group than that in the control group. Renalase expression was decreased in the kidney after 60 min of exercise, whereas the expression of renalase mRNA and protein in the extensor digitorum longus and plantaris muscles, respectively, increased after exercise. However, the expression of renalase in the other tissues examined did not change after acute exercise. In conclusion, we report that MEX for 60 min increases both renalase concentration in the blood and its expression in skeletal muscle.

中文翻译：

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中等强度的运动会增加大鼠血液和骨骼肌中的肾酶水平。

肾病酶主要在肾脏中表达，在儿茶酚胺代谢和血压调节中发挥作用。中等强度运动（MEX）已显示可增加血液中肾脏酶的浓度并降低人的肾功能。此外，据报道这种运动会增加儿茶酚胺水平。在这里，我们检查了大鼠MEX后血液中肾酶浓度和不同器官中肾酶表达水平。使12只雄性Wistar大鼠在跑步机（MEX组）上以20 m·min ^-1的速度运行60分钟。，休息15分钟后。将对照组大鼠放在跑步机上安乐死。使用蛋白质印迹，实时RT-PCR和ELISA分析组织和血液样品。总体而言，MEX组的血液中肾脏酶浓度明显高于对照组。运动60分钟后，肾脏中的肾酶酶表达降低，而运动后，指趾长伸肌和plant肌中肾酶mRNA和蛋白的表达分别升高。但是，急性运动后，其他检查组织中肾酶的表达没有改变。总之，我们报告MEX 60分钟会增加血液中的肾脏酶浓度及其在骨骼肌中的表达。