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All-fibre supercontinuum laser for in vivo multispectral photoacoustic microscopy of lipids in the extended near-infrared region.
Photoacoustics ( IF 7.9 ) Pub Date : 2020-01-27 , DOI: 10.1016/j.pacs.2020.100163
Manoj K Dasa 1 , Gianni Nteroli 2 , Patrick Bowen 3 , Giulia Messa 4 , Yuyang Feng 5 , Christian R Petersen 1, 6 , Stella Koutsikou 4 , Magalie Bondu 3 , Peter M Moselund 3 , Adrian Podoleanu 2 , Adrian Bradu 2 , Christos Markos 1, 6 , Ole Bang 1, 3, 6
Affiliation  

Among the numerous endogenous biological molecules, information on lipids is highly coveted for understanding both aspects of developmental biology and research in fatal chronic diseases. Due to the pronounced absorption features of lipids in the extended near-infrared region (1650−1850 nm), visualisation and identification of lipids become possible using multi-spectral photoacoustic (optoacoustic) microscopy. However, the spectroscopic studies in this spectral region require lasers that can produce high pulse energies over a broad spectral bandwidth to efficiently excite strong photoacoustic signals. The most well-known laser sources capable of satisfying the multi-spectral photoacoustic microscopy requirements (tunability and pulse energy) are tunable nanosecond optical parametric oscillators. However, these lasers have an inherently large footprint, thus preventing their use in compact microscopy systems. Besides, they exhibit low-repetition rates. Here, we demonstrate a compact all-fibre, high pulse energy supercontinuum laser that covers a spectral range from 1440 to 1870 nm with a 7 ns pulse duration and total energy of 18.3 μJ at a repetition rate of 100 kHz. Using the developed high-pulse energy source, we perform multi-spectral photoacoustic microscopy imaging of lipids, both ex vivo on adipose tissue and in vivo to study the development of Xenopus laevis tadpoles, using six different excitation bands over the first overtone transition of C–H vibration bonds (1650−1850 nm).



中文翻译:

全光纤超连续谱激光,用于在体内扩展的近红外区域中的脂质体内多光谱光声显微镜。

在众多内源性生物分子中,关于脂质的信息被人们垂涎,以了解发育生物学和致命性慢性病研究的两个方面。由于脂质在扩展的近红外区域(1650-1850 nm)中具有明显的吸收特征,因此使用多光谱光声(光声)显微镜可以对脂质进行可视化和鉴定。然而,在该光谱区域中的光谱研究需要能够在宽光谱带宽上产生高脉冲能量的激光器,以有效地激发强光声信号。能够满足多光谱光声显微镜要求(可调性和脉冲能量)的最著名的激光源是可调纳秒光学参量振荡器。然而,这些激光器具有固有的占地面积大,因此无法在紧凑型显微镜系统中使用。此外,它们显示出低重复率。在这里,我们展示了一种紧凑的全光纤,高脉冲能量超连续谱激光器,该激光器以1100 kHz的重复频率覆盖了1440至1870 nm的光谱范围,脉冲持续时间为7 ns,总能量为18.3μJ。使用发达的高脉冲能量源,我们对脂质进行多光谱光声显微镜成像,体外对脂肪组织和体内研究的发展非洲爪蟾蝌蚪,使用六种不同的激发带超过C-H振动键的第一泛音过渡(1650年至1850年纳米)。

更新日期:2020-01-27
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