Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based on the conservation of aging across tissues and taking advantage of the relatively well-characterization of the myofibers and associated SCs, skeletal muscle emerged as an experimental system to study the decline in function and maintenance of old tissues and to explore rejuvenation strategies. In this review, we summarize the approaches for understanding the aging process and for assaying the success of rejuvenation that use skeletal muscle as the experimental system of choice. We further discuss (and exemplify with studies of skeletal muscle) how conflicting results might be due to variations in the techniques of stem cell isolation, differences in the assays of functional rejuvenation, or deciding on the numbers of replicates and experimental cohorts.

中文翻译：

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骨骼肌作为研究组织老化和再生的首选实验模型。

骨骼肌是哺乳动物中对年龄最敏感的组织之一。其驻留干细胞（即卫星细胞，SC）、分化细胞（即肌纤维）和细胞外基质的显着变化导致组织稳态、功能和再生能力下降。基于对跨组织老化的保护，并利用肌纤维和相关 SCs 相对较好的表征，骨骼肌成为了一个实验系统，用于研究旧组织功能下降和维持，并探索年轻化策略。在这篇综述中，我们总结了理解衰老过程和分析使用骨骼肌作为所选实验系统的年轻化成功的方法。