The feasibility of magnetic levitational bioassembly of tissue-engineered constructs from living tissue spheroids in the presence of paramagnetic ions (i.e. Gd 3+ ) was recently demonstrated. However, Gd 3+ is relatively toxic at concentrations above 50 mM normally used to enable magnetic levitation with NdFeB-permanent magnets. Using a high magnetic field (a 50 mm-bore, 31 T Bitter magnet) at the High Field Magnet Laboratory at Radboud University in Nijmegen, The Netherlands, we performed magnetic levitational assembly of tissue constructs from living spheroids prepared from the SW1353 chondrosarcoma cell line at 0.8 mM Gd 3+ containing salt gadobutrol at 19 T magnetic field. The parameters of the levitation process were determined on the basis of polystyrene beads with a 170 μ m-diameter. To predict the theoretical possibility of assembly, a zone of stable levitation in the horizontal and vertical areas of cross sections was previously calcul...

中文翻译：

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在高磁场中使用悬浮组装的无支架和无标签生物制造技术。

最近证明了在顺磁离子（即 Gd 3+ ）存在下从活组织球体中进行组织工程化构建体的磁悬浮生物组装的可行性。然而，Gd 3+ 在浓度高于 50 mM 时具有相对毒性，通常用于使用 NdFeB 永磁体实现磁悬浮。我们在荷兰奈梅亨 Radboud 大学的高场磁铁实验室使用高磁场（50 毫米孔径、31 T 苦味磁铁）对 SW1353 软骨肉瘤细胞系制备的活球体组织构建体进行磁悬浮组装0.8 mM Gd 3+ 含盐钆布醇，磁场为 19 T。悬浮过程的参数是在直径为 170 μ m 的聚苯乙烯珠粒的基础上确定的。为了预测组装的理论可能性，