Abstract

The synthesis of heat shock protein 70 (HSP70) is temperature-dependent and its response is considered as a cellular thermometer in response to thermal stress. The variation in HSP70 gene expression has been positively correlated with thermo-tolerance. Three different goat populations (Black Bengal, Ganjam and Raighar) which differ in body size, coat color and production performance were assessed for effect of thermal stress at different temperature humidity index (THI). The physiological vital parameters like rectal temperature (RT), skin temperature (ST), heart rate (HR) and respiration rate (RR) which are related to thermal stress susceptibility were recorded. The genetic variation of HSP70 gene in the three goat populations and its effect on physiological vital parameters related to thermal stress was assessed. A novel polymorphism (C241T) in the exonic region of HSP70 gene with significant (p < .05) statistical association with the physiological vital parameters like RT, ST and RR was identified indicating its role in thermo-tolerance.

• Highlights

• The HSP70 gene was found to be polymorphic in Indian goats.

• The non-synonymous nucleotide change (C241T) resulted change in amino acid Arginine 241 Cysteine in mature polypeptide which were not reported earlier.

• The constructed phylogenetic tree showed that Ganjam and Raighar goats are more close to each other.

摘要

热休克蛋白 70 (HSP70) 的合成依赖于温度，其响应被认为是响应热应力的细胞温度计。HSP70基因表达的变异与耐热性呈正相关。评估了三种不同的山羊种群（Black Bengal、Ganjam 和 Raighar），它们在不同的温度湿度指数 (THI) 下评估了热应激对体型、毛色和生产性能的影响。记录直肠温度（RT）、皮肤温度（ST）、心率（HR）和呼吸率（RR）等与热应激敏感性相关的生理生命参数。HSP70的遗传变异评估了三个山羊种群中的基因及其对与热应激相关的生理重要参数的影响。在HSP70基因的外显子区域中发现了一种新的多态性 (C241T)， 与生理学重要参数如 RT、ST 和 RR具有显着的 ( p < .05) 统计关联，表明其在耐热性中的作用。

• 强调

• 该HSP70基因被发现在印度山羊多态。

• 非同义核苷酸变化 (C241T) 导致成熟多肽中氨基酸精氨酸 241 半胱氨酸的变化，这是早先未报道的。

• 构建的系统发育树表明 Ganjam 和 Raighar 山羊彼此更接近。