Ureteric progenitor cells (UPCs) within the branch tips of the arborizing ureteric epithelium of the kidney's developing collecting system establish the shape and cellular organization of the collecting network, and drive the nephrogenic program through their interactions with nephron progenitor cells. In a previous study, expression screening identified a cohort of genes showing UPC‐enriched expression including D17H6S56E‐5 , Hs3st3a1 , Hs3st3b1 , and Tmem59l . Each of these is also enriched in branch tips of assembling airways of the developing lungs. Here, we used Crispr‐CAS9 directed gene editing to mutate each of these targets to address their potential role(s) in UPC programs.

中文翻译：

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在小鼠肾脏分支形态发生中具有输尿管祖细胞特异性表达的基因的突变分析。

肾脏发育中的采集系统的呈树状输尿管上皮的分支尖端内的输尿管祖细胞（UPC）建立了收集网络的形状和细胞组织，并通过与肾单位祖细胞的相互作用来驱动肾生成程序。在先前的研究中，表达筛选确定了一组显示UPC富集表达的基因，包括D17H6S56E-5，Hs3st3a1，Hs3st3b1和Tmem59l。这些中的每一个也丰富了正在发育的肺部的气道的分支尖端。在这里，我们使用了Crispr-CAS9指导的基因编辑来突变这些靶标中的每一个，以解决它们在UPC程序中的潜在作用。