The storage of anaerobic ammonia oxidizing bacteria (anammox) plays an important role in the application of anammox. Glycerol, sodium alginate and DMSO were used as the cryoprotectant, and vacuum lyophilization was used to prepare the anammox bacteria powder. Simultaneously, the control experiment was set up with the same protectant and preservation time. Bacteria powders were preserved using vacuum lyophilization and preserved at 4 °C for 60 days. During the 54 days of rejuvenation, the reactors that were inoculated with bacteria powder preserved by different methods showed significant difference. The results show that the anammox bacteria powder with 3 wt% DMSO as the cryoprotectant and without the substrate solution presented the best rejuvenation effect. The average specific anammox activity was 115.84 mg-N·(g VSS·d)-1 with an activity recovery rate of 89%, and its stoichiometric ratio (Rs and Rp) was 1.33 and 0.21, which were very close to the theoretical values. The vacuum lyophilization method for the long-term preservation of anammox bacteria was effective.

中文翻译：

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厌氧菌的真空冷冻干燥保存和再生性能。

厌氧氨氧化细菌（anammox）的储存在厌氧菌的应用中起着重要作用。使用甘油，海藻酸钠和DMSO作为冷冻保护剂，并真空冷冻干燥以制备厌氧氨氧化菌粉末。同时，建立了具有相同保护和保存时间的对照实验。细菌粉末用真空冷冻干燥保存，并在4℃保存60天。在恢复活力的54天中，用不同方法保存的细菌粉末接种的反应器显示出显着差异。结果表明，以3wt％DMSO作为冷冻保护剂且无底物溶液的厌氧菌粉末具有最佳的复兴效果。平均厌氧氨氧化比活度为115。84 mg-N·（g VSS·d）-1的活度恢复率为89％，化学计量比（Rs和Rp）为1.33和0.21，非常接近理论值。真空冷冻干燥法可长期保存厌氧氨氧化菌。