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A novel GH30 xylobiohydrolase from Acremonium alcalophilum releasing xylobiose from the non-reducing end
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.enzmictec.2019.109484
Katarína Šuchová 1 , Vladimír Puchart 1 , Nikolaj Spodsberg 2 , Kristian B R Mørkeberg Krogh 2 , Peter Biely 1
Affiliation  

Xylanases of the GH30 family are grouped to subfamilies GH30-7 and GH30-8. The GH30-8 members are of bacterial origin and well characterized, while the GH30-7 members are from fungal sources and their properties are quite diverse. Here, a heterologous expression and characterization of the GH30-7 xylanase AaXyn30A from a cellulolytic fungus Acremonium alcalophilum is reported. From various polymeric and oligomeric substrates AaXyn30A generates xylobiose as the main product. It was proven that xylobiose is released from the non-reducing end of all tested substrates, thus the enzyme behaves as a typical non-reducing-end acting xylobiohydrolase. AaXyn30A is active on different types of xylan, exhibiting the highest activity on rhodymenan (linear β-1,3-β-1,4-xylan) from which also an isomeric xylotriose Xyl-β-1,3-Xyl-β-1,4-Xyl is formed. Production of xylobiose from glucuronoxylan is at later stage accompanied by a release of aldouronic acids differing from those liberated by the bacterial GH30-8 glucuronoxylanases.

中文翻译:

来自 Acremonium alcalophilum 的新型 GH30 木二糖水解酶从非还原端释放木二糖

GH30 家族的木聚糖酶分为亚家族 GH30-7 和 GH30-8。GH30-8 成员是细菌来源且特征明确,而 GH30-7 成员来自真菌来源,它们的特性非常多样化。在这里,报道了来自纤维素分解真菌 Acremonium alcalophilum 的 GH30-7 木聚糖酶 AaXyn30A 的异源表达和表征。AaXyn30A 从各种聚合和寡聚底物生成木二糖作为主要产物。已证明木二糖是从所有测试底物的非还原端释放出来的,因此该酶表现为典型的非还原端作用木二糖水解酶。AaXyn30A 对不同类型的木聚糖具有活性,对红木聚糖(线性 β-1,3-β-1,4-木聚糖)表现出最高的活性,其中还有一种异构木三糖 Xyl-β-1,3-Xyl-β-1 ,4-Xyl 形成。
更新日期:2020-03-01
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