Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-d-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.

中文翻译：

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一种新型芒果苷的酶促合成及生物学表征

芒果苷是 Mangifera indica L. 的主要成分，因其抗氧化、抗糖尿病、抗炎和抗微生物活性而备受关注。然而，它在水中的溶解度差限制了它在食品和制药行业的应用。在本研究中，使用来自肠系膜明串珠菌 B-512F/KM 的葡聚糖蔗糖酶，从芒果苷和蔗糖酶法合成了新型芒果苷-(1→6)-α-d-吡喃葡萄糖苷 (Mg-G1)，并使用响应面方法进行了优化。发现 Mg-G1 的水溶性为 824.7 mM，比芒果苷高 2300 多倍。Mg-G1 还显示出 DPPH 自由基清除活性和超氧化物歧化酶 (SOD) 样清除活性，分别比芒果苷高 4.77 和 3.71 倍。Mg-G1 显示出对人肠道麦芽糖酶和 COX-2 的抑制活性。因此，新的葡糖基化芒果苷可用作功能性食品和药物应用中的成分。