This study investigates the twitching ability of 28 clinical and five environmental strains of S. maltophilia grown under iron-depleted condition through in-silico, phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets of type IV pilus shared across S. maltophilia strains K279a, R551-3, D457 and JV3. The macroscopic twitching assay showed that only clinical isolates produced a zone of twitching with a mean of 22.00 mm under normal and 25.00 mm under iron-depleted conditions. (p = 0.002). Environmental isolates did not show any significant twitching activity in both conditions tested. Isobaric Tags for Relative and Absolute Quantification (ITRAQ) analysis showed altered expression of twitching motility protein PilT (99.08-fold change), flagellar biosynthesis protein FliC (20.14-fold change), and fimbrial protein (0.70-fold change) in response to iron-depleted condition. Most of the strains that have the ability to twitch under the normal condition, exhibit enhanced twitching during iron limitation.

中文翻译：

---

铁限制下嗜麦芽寡养单胞菌的抽搐运动：计算机、表型和蛋白质组学方法。

本研究通过计算机、表型和蛋白质组学方法研究了在缺铁条件下生长的 28 种临床和 5 种环境嗜麦芽糖链球菌菌株的抽搐能力。使用子系统技术 (RAST) 分析的快速注释揭示了嗜麦芽糖链球菌菌株 K279a、R551-3、D457 和 JV3 共有 21 个 IV 型菌毛靶标的存在。宏观抽搐测定表明，只有临床分离株才会产生抽搐区域，正常情况下平均为 22.00 毫米，缺铁条件下平均为 25.00 毫米。（p = 0.002）。在两种测试条件下，环境分离株均未表现出任何明显的抽搐活动。用于相对和绝对定量 (ITRAQ) 分析的同量异位标签显示，抽搐运动蛋白 PilT（99.08 倍变化）、鞭毛生物合成蛋白 FliC（20.14 倍变化）和菌毛蛋白（0.70 倍变化）的表达因铁反应而改变- 耗尽状态。大多数在正常条件下具有抽搐能力的菌株在铁限制期间表现出增强的抽搐。