Advanced developments in the field of enzyme technology have increased the use of enzymes in industrial applications, especially in detergents. Enzymes as detergent additives have been extensively studied and the demand is considerably increasing due to its distinct properties and potential applications. Enzymes from microorganisms colonized at various geographical locations ranging from extreme hot to cold are explored for compatibility studies as detergent additives. Especially psychrophiles growing at cold conditions have cold-active enzymes with high catalytic activity and their stability under extreme conditions makes it as an appropriate eco-friendly and cost-effective additive in detergents. Adequate number of reports are available on cold-active enzymes such as proteases, lipases, amylases, and cellulases with high efficiency and exceptional features. These enzymes with increased thermostability and alkaline stability have become the premier choice as detergent additives. Modern approaches in genomics and proteomics paved the way to understand the compatibility of cold-active enzymes as detergent additives in broader dimensions. The molecular techniques such as gene coding, amino acid sequencing, and protein engineering studies helped to solve the mysteries related to alkaline stability of these enzymes and their chemical compatibility with oxidizing agents. The present review provides an overview of cold-active enzymes used as detergent additives and molecular approaches that resulted in development of these enzymes as commercial hit in detergent industries. The scope and challenges in using cold-active enzymes as eco-friendly and sustainable detergent additive are also discussed.

中文翻译：

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当前预期将冷活性酶用作环保洗涤剂添加剂。

酶技术领域的先进发展增加了酶在工业应用中的使用，尤其是在洗涤剂中。作为去污剂添加剂的酶已被广泛研究，由于其独特的性能和潜在的应用，需求大大增加。探索了在极端炎热到寒冷等各种地理位置定居的微生物酶作为洗涤剂添加剂的相容性研究。尤其是在寒冷条件下生长的嗜冷菌具有具有高催化活性的冷活性酶，其在极端条件下的稳定性使其成为洗涤剂中合适的生态友好型和高性价比的添加剂。关于冷活性酶（如蛋白酶，脂肪酶，淀粉酶，纤维素酶效率高，功能卓越。这些具有增强的热稳定性和碱性稳定性的酶已成为洗涤剂添加剂的首选。基因组学和蛋白质组学的现代方法为了解冷活性酶作为洗涤剂添加剂在更大范围内的相容性铺平了道路。诸如基因编码，氨基酸测序和蛋白质工程研究等分子技术帮助解决了与这些酶的碱性稳定性及其与氧化剂的化学相容性有关的奥秘。本综述概述了用作洗涤剂添加剂的冷活性酶和分子方法，这些酶导致了这些酶在洗涤剂工业中的商业应用。