INTRODUCTION Density and co-expression of tumor necrosis factor (TNF) receptors may vary among cell populations. However, the role and potential of these changes remain unclear. This study aimed to determine the density of expression and co-expression of TNFR1/2 and the dose-dependent effect of soluble TNF on these parameters. METHODS Epithelial-like (HEp-2, K-562, MCF-7, ZR-75/1) and lymphoblast-like (MOLT-4, HL-60, Raji, RPMI-8226, IM-9) cell lines were characterized for co-expression of TNFR1/2 using a modified flow cytometry protocol. The dose-dependent effects of rhTNF on TNF receptor expression in these lines were studied. RESULTS This study reports a protocol for the simultaneous quantitative evaluation of the of TNF receptor number and co-expression of membrane-bound TNFR1/2. Cells within one tumor cell line were found to differ regarding their expression of type 1 and 2 TNFα receptors; simultaneously, cells with all 4 variants of co-expression may be present in culture. CONCLUSION We demonstrated a dose-dependent effect of TNF on changes in the expression of TNFR1/2 by the percentage of positive cells and by the number of receptors, which may be used to control TNF-mediated processes in target cells.

中文翻译：

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通过肿瘤细胞系共表达膜结合的肿瘤坏死因子-α受体1和2。

引言肿瘤坏死因子（TNF）受体的密度和共表达可能随细胞群体而变化。但是，这些变化的作用和潜力尚不清楚。这项研究旨在确定TNFR1 / 2的表达和共表达的密度以及可溶性TNF对这些参数的剂量依赖性作用。方法鉴定上皮样（HEp-2，K-562，MCF-7，ZR-75 / 1）和淋巴母细胞样（MOLT-4，HL-60，Raji，RPMI-8226，IM-9）细胞系使用改良的流式细胞仪方案共表达TNFR1 / 2。研究了rhTNF对这些系中TNF受体表达的剂量依赖性作用。结果本研究报告了同时定量评估TNF受体数量和膜结合TNFR1 / 2共表达的方案。发现一种肿瘤细胞系中的细胞在1型和2型TNFα受体的表达方面有所不同。同时，培养物中可能存在具有全部4种共表达变体的细胞。结论我们证明了TNF对TNFR1 / 2表达的影响具有剂量依赖性，其作用是通过阳性细胞的百分比和受体的数量来实现的，这些作用可用于控制靶细胞中TNF介导的过程。