In Drosophila, the deposition of the germ plasm at the posterior pole of the oocyte is essential for the abdomen and germ cell formation during embryogenesis. To assemble the germ plasm, oskar (osk) mRNA, produced by nurse cells, should be localized and anchored on the posterior cortex of the oocyte. Processing bodies (P-bodies) are cytoplasmic RNA granules responsible for the 5'-3' mRNA degradation. Evidence suggests that the components of P-bodies, such as Drosophila decapping protein 1 and Ge-1, are involved in the posterior localization of osk. However, whether the decapping core enzyme, Drosophila decapping protein 2 (dDcp2), is also involved remains unclear. Herein, we generated a dDcp2 null allele and showed that dDcp2 was required for the posterior localization of germ plasm components including osk. dDcp2 was distributed on the oocyte cortex and was localized posterior to the osk. In the posterior pole of dDcp2 mutant oocytes, osk was mislocalized and colocalized with F-actin detached from the cortex; moreover, considerably fewer F-actin projections were observed. Using the F-actin cosedimentation assay, we proved that dDcp2 interacted with F-actin through its middle region. In conclusion, our findings explored a novel function of dDcp2 in assisting osk localization by modulating the formation of F-actin projections on the posterior cortex.

中文翻译：

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果蝇开盖蛋白2调节皮层F-肌动蛋白的形成，促进种质组装。

在果蝇中，种质在卵母细胞后极的沉积对于胚胎发生过程中腹部和生殖细胞的形成至关重要。要组装种质，应将哺乳细胞产生的oskar（osk）mRNA定位并锚定在卵母细胞的后皮质。加工体（P体）是负责5'-3'mRNA降解的细胞质RNA颗粒。有证据表明，P体的成分，如果蝇去壳蛋白1和Ge-1，参与了osk的后定位。但是，还不清楚是否还涉及去壳核心酶果蝇去壳蛋白2（dDcp2）。在这里，我们生成了dDcp2无效等位基因，并表明dDcp2是包括osk在内的种质成分后定位所必需的。dDcp2分布在卵母细胞皮层上，位于骨后方。在dDcp2突变卵母细胞的后极，osk错位并与从皮层分离的F-肌动蛋白共定位。此外，观察到的F-肌动蛋白投影明显更少。使用F-肌动蛋白沉淀实验，我们证明dDcp2通过其中间区域与F-肌动蛋白相互作用。总之，我们的发现探索了dDcp2通过调节后皮质F-肌动蛋白突起的形成来协助osk定位的新功能。我们证明了dDcp2通过其中间区域与F-肌动蛋白相互作用。总之，我们的发现探索了dDcp2通过调节后皮质F-肌动蛋白突起的形成来协助osk定位的新功能。我们证明了dDcp2通过其中间区域与F-肌动蛋白相互作用。总之，我们的发现探索了dDcp2通过调节后皮层F-肌动蛋白突起的形成来协助osk定位的新功能。