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Molecular identification of novel and genetically diverse adenoviruses in Passeriform birds.
Virus Genes ( IF 1.6 ) Pub Date : 2020-02-07 , DOI: 10.1007/s11262-020-01739-3
Monika Rinder 1 , Anna Schmitz 2 , Noreen Baas 1 , Rüdiger Korbel 1
Affiliation  

Knowledge about adenoviruses in birds of the order Passeriformes is very scarce. Based on molecular characterizations, only two siadenoviruses, great tit adenovirus 1 and Gouldian finch adenovirus, have been described so far occurring in great tits and Gouldian finches, respectively. Assuming a broader occurrence of adenoviruses, various passeriform birds including pet, zoo, and wild birds were examined using a broad-range PCR targeting a fragment of the adenovirus DNA polymerase gene. Adenoviruses were detected in 25 individual birds belonging to 13 species and seven zoological families (Ploceidae, Fringillidae, Estrildidae, Paridae, Sylviidae, Turdidae, Muscicapidae). The putative viruses were further characterized by sequencing the PCR products and phylogenetic analyses. DNA of adenoviruses affiliating to 3 genera including aviadenovirus, siadenovirus, and atadenovirus was found. Viruses with sequences identical or closely related to great tit adenovirus 1 and Gouldian finch adenovirus 1 were detected in a great tit and in two zebra finches, respectively. Based on polymerase amino acid sequence comparisons, the viruses found in the remaining 22 birds revealed phylogenetic distances larger than 15% to adenoviruses known so far suggesting that they may belong to at least 14 different virus species. In some bird species (great tit, zebra finch, vitelline masked weaver) varying adenovirus genera were detected. These results suggest a broad variety of adenoviruses circulating in passeriform birds.

中文翻译:

在Passeriform鸟类中新型和遗传多样的腺病毒的分子鉴定。

关于雀形目鸟类的腺病毒的知识非常匮乏。基于分子特征,到目前为止,仅描述了两种唾液腺病毒,即大山雀腺病毒1和古氏朱雀腺病毒,分别发生在大山雀和古朱雀中。假定腺病毒的发生范围更广,使用靶向腺病毒DNA聚合酶基因片段的广谱PCR检测了包括宠物,动物园和野生鸟类在内的各种鸟形鸟类。在属于13个物种和7个动物学科(P科,F科,Est科,Par科,S科,Tur科,Mus科)的25只个体鸟类中检测到腺病毒。通过对PCR产物进行测序和系统发育分析来进一步表征推定的病毒。与3属有关的腺病毒的DNA,包括腺病毒,发现了唾液腺病毒和腺病毒。在大山雀和两个斑马雀中分别检测到具有与大山雀腺病毒1和古尔芬雀科腺病毒1相同或密切相关的序列的病毒。根据聚合酶氨基酸序列比较,在其余22只禽鸟中发现的病毒与迄今为止已知的腺病毒相比,系统发育距离大于15%,这表明它们可能属于至少14种不同的病毒种类。在某些鸟类中(大山雀,斑马雀科,卵黄膜遮蔽的韦弗),检测到各种腺病毒属。这些结果表明,在鸟形鸟中循环的各种各样的腺病毒。在大山雀和两个斑马雀中分别检测到具有与大山雀腺病毒1和古尔芬雀科腺病毒1相同或密切相关的序列的病毒。根据聚合酶氨基酸序列比较,在其余22只禽类中发现的病毒与迄今为止已知的腺病毒相比,系统发育距离大于15%,这表明它们可能属于至少14种不同的病毒种类。在某些鸟类中(大山雀,斑马雀科,卵黄膜遮蔽的韦弗),检测到各种腺病毒属。这些结果表明,在鸟形鸟中循环的各种各样的腺病毒。在大山雀和两个斑马雀中分别检测到具有与大山雀腺病毒1和古尔芬雀科腺病毒1相同或密切相关的序列的病毒。根据聚合酶氨基酸序列比较,在其余22只禽鸟中发现的病毒与迄今为止已知的腺病毒相比,系统发育距离大于15%,这表明它们可能属于至少14种不同的病毒种类。在某些鸟类中(大山雀,斑马雀科,卵黄膜遮蔽的韦弗),检测到各种腺病毒属。这些结果表明,在鸟形鸟中循环的各种各样的腺病毒。在剩下的22只鸟类中发现的病毒与迄今为止已知的腺病毒相比,系统发育距离超过15%,这表明它们可能属于至少14种不同的病毒种类。在某些鸟类中(大山雀,斑马雀科,卵黄膜遮蔽的韦弗),检测到各种腺病毒属。这些结果表明,在鸟形鸟中循环的各种各样的腺病毒。在剩下的22只鸟类中发现的病毒与迄今为止已知的腺病毒相比,系统发育距离大于15%,这表明它们可能属于至少14种不同的病毒种类。在某些鸟类中(大山雀,斑马雀科,卵黄膜遮蔽的韦弗),检测到各种腺病毒属。这些结果表明,在鸟形鸟中循环的各种各样的腺病毒。
更新日期:2020-02-07
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