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Characterization of the First mecA-Positive Multidrug-Resistant Staphylococcus pseudintermedius Isolated from an Argentinian Patient.
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2020-07-07 , DOI: 10.1089/mdr.2019.0308
Paula Gagetti 1 , Laura Errecalde 2 , Alice R Wattam 3 , Denise De Belder 1 , Matthew Ojeda Saavedra 4 , Alejandra Corso 1 , Adriana E Rosato 4
Affiliation  

Staphylococcus pseudintermedius is commonly associated with colonization or infection in dogs, and was identified as a novel species within the genus Staphylococcus in 2006. Methicillin resistance emerged in S. pseudintermedius during the last decade. We describe here a genomic characterization of the first methicillin-resistant S. pseudintermedius (MRSP) recovered from a human patient in Argentina. The strain was phenotypically identified as MRSP 8510 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and antimicrobial susceptibility testing. We assessed genetic characterization by mecA PCR, SCCmec (staphylococcal chromosomal cassette) typing, and whole-genome sequencing. MRSP 8510 was phenotypically resistant to six classes of antimicrobial agents, consistent with the genes found in its genome. We concluded that MRSP 8510 was a multidrug-resistant ST1412 isolate. This study highlights the importance of the detection and characterization of pathogens with potential risks of zoonotic transmission to humans, as they may constitute a reservoir of genes associated with antimicrobial resistance.

中文翻译:

从阿根廷患者中分离出的第一个mecA阳性多药耐药葡萄球菌pseudintermedius的特征。

pseudintermedius葡萄球菌通常与狗的定植或感染有关,在2006年被鉴定为Staphylococcus属中的一个新物种。最近十年,S。pseudintermedius出现了对甲氧西林的耐药性。我们在这里描述了从阿根廷的一名人类患者中回收的第一个耐甲氧西林的伪中间链霉菌(MRSP)的基因组特征。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)和抗菌药敏试验,在表型上将该菌株鉴定为MRSP 8510。我们通过mecA PCR,SCC mec评估了遗传特征(葡萄球菌染色体盒)分型和全基因组测序。MRSP 8510对六类抗菌剂具有表型抵抗力,与其在基因组中发现的基因一致。我们得出的结论是,MRSP 8510是耐多药的ST1412分离株。这项研究强调了对具有人畜共患病传染给人类的潜在风险的病原体进行检测和表征的重要性,因为它们可能构成与抗菌素耐药性相关的基因库。
更新日期:2020-07-10
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