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Distribution of carbapenem resistant Acinetobacter baumannii with blaADC-30 and induction of ADC-30 in response to beta-lactam antibiotics.
Research in Microbiology ( IF 2.6 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.resmic.2020.01.002
Birson Ingti 1 , Supriya Upadhyay 2 , Monalisha Hazarika 3 , Annie Bakorlin Khyriem 4 , Deepjyoti Paul 3 , Prithwis Bhattacharya 5 , S R Joshi 2 , Debajyoti Bora 6 , Debadatta Dhar 7 , Amitabha Bhattacharjee 3
Affiliation  

A wide range of intrinsic Acinetobacter-derived cephalosporinases (ADC) along with other carbapenemases has now been detected in Acinetobacter baumannii leaving clinicians with few treatment options. The present study reports the spread of ADC-30 co-producing KPC-2 along with other β-lactamases among carbapenem resistant A. baumannii strains obtained from ICU patients in two Indian hospitals. Primer extension analysis revealed higher transcript level of the ADC gene when induced with cefoxitin at 8 μg/ml (170 fold), ceftriaxone at 8 μg/ml (136 fold), ceftazidime at 4 μg/ml (65 fold), cefepime at 8 μg/ml (77 fold) and aztreonam at 8 μg/ml (21 fold) when compared with the basal level without antibiotic pressure. Slight increase in expression of blaADC-30 when induced with imipenem and meropenem at 0.25 μg/ml (3 and 6 fold) was observed and may help in conferring resistance to carbapenem. MLST analysis revealed the circulation of A. baumannii sequence types ST188, ST386, ST583 and ST390 in these hospitals.



中文翻译:

带有blaADC-30的耐碳青霉烯的鲍曼不动杆菌的分布以及对β-内酰胺类抗生素的响应引起ADC-30的诱导。

现已在鲍曼不动杆菌中检测到多种内源性不动杆菌源性头孢菌素酶(ADC)以及其他碳青霉烯酶,因此临床医生几乎没有治疗选择。本研究报道了在两家印度医院从ICU患者那里获得的耐碳青霉烯鲍曼不动杆菌菌株中,ADC-30共同产生的KPC-2和其他β-内酰胺酶的传播。引物延伸分析显示,当以头孢西丁8μg/ ml(170倍),头孢曲松钠8μg/ ml(136倍),头孢他啶4μg/ ml(65倍),头孢吡肟8克时,ADC基因的转录水平更高与无抗生素压力的基础水平相比,微克/毫升(77倍)和氨曲南为8微克/毫升(21倍)。bla表达略有增加当用亚胺培南和美洛培南以0.25μg/ ml(3倍和6倍)诱导时,ADC-30被观察到,可能有助于赋予对碳青霉烯的抗性。MLST分析显示这些医院中鲍曼不动杆菌序列类型ST188,ST386,ST583和ST390的流通。

更新日期:2020-01-25
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