This study was performed to evaluate the effects of dietary probiotics on growth, non‐specific immune responses and disease resistance in olive flounder, Paralichthys olivaceus. During 8 weeks, the fish were fed the five experimental diets such as a basal commercial diet (CON), oxytetracycline (OTC) and three basal diets containing Bacillus subtilis (BS), a commercial microbial product (CES) and a mixture of yeast and bacterium (PI), respectively. Fish fed all the probiotics diets and OTC showed a significantly higher growth than fish‐fed CON (P < 0·05). Fish‐fed PI had a significantly higher nitroblue tetrazolium activity, whereas fish‐fed CES showed a higher lysozyme level (P < 0·05). A 7‐day challenge test also showed that fish‐fed PI had a cumulative survival rate equivalent to that of fish‐fed OTC (P < 0·05). Moreover, the diet (PI) appeared to increase the diversity of microbial community in the fish. All these results suggest that the probiotics diet could function as a potential antibiotic replacer in the olive flounder.

中文翻译：

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Groenewaldozyma salmanticensis 和 Gluconacetobacter liquefaciens 的混合物对Paralichthys olivaceus 生长和免疫反应的益生菌作用

本研究旨在评估膳食益生菌对橄榄比目鱼 Paralichthys olivaceus 的生长、非特异性免疫反应和抗病性的影响。在 8 周内，给鱼喂食五种实验饮食，例如基础商业饮食 (CON)、土霉素 (OTC) 和三种含有枯草芽孢杆菌 (BS)、商业微生物产品 (CES) 以及酵母和酵母菌混合物的基础饮食。细菌（PI），分别。喂食所有益生菌饮食和 OTC 的鱼比喂鱼的 CON 显示出显着更高的生长（P < 0·05）。喂鱼的 PI 具有显着更高的硝基蓝四唑活性，而喂鱼的 CES 显示出更高的溶菌酶水平（P < 0·05）。一项为期 7 天的激发试验还表明，鱼饲 PI 的累积存活率与鱼饲 OTC 相当（P < 0·05）。而且，饮食（PI）似乎增加了鱼中微生物群落的多样性。所有这些结果表明，益生菌饮食可以作为橄榄比目鱼的潜在抗生素替代品。