During the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized the diagnosis of fungal infections. Recently, a new Conidia ID-fungi plate (IDFP) medium was introduced to facilitate growth and sampling of fungi. This study aimed to evaluate the IDFP for fungal MALDI-TOF MS identification by comparison with a standard fungal growth medium using two reference libraries. A total of 75 filamentous fungal isolates (including 32 dermatophytes) were inoculated on IDFP and Sabouraud-gentamicin-chloramphenicol (SGC) agar and identified by MALDI-TOF MS using formic acid/acetonitrile extraction. Both the commercially available Bruker library (version 2.0) and the public available MSI web application (version 2018) were applied. For 15% of the isolates, a faster growth was noticed on IDFP compared to SGC. IDFP enhanced the performance of fungal identification compared to SGC for both MSI (increase of 16% identifications to genus and 5% to species level) and Bruker library (increase of 22% identifications to genus and 8% to species level). In total, only 73% of the tested isolates were present in the Bruker library compared to 92% for MSI library. No significant difference (P = 0.46) in MALDI score between IDFP and SGC was observed for the MSI library, but scores were significantly (P = 0.03) higher for IDFP when using Bruker library, potentially explained by the prevention of agar contamination by using IDFP since the Bruker database was created from liquid media. IDFP is a promising alternative growth medium for MALDI-TOF MS fungal identification which would strongly benefit from optimizing the Bruker reference library.

中文翻译：

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使用两种类型的参考库（Bruker和MSI）通过Bruker MALDI Biotyper鉴定真菌，从而可以对新型ID-真菌板进行性能测试。

在过去的十年中，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）彻底改变了真菌感染的诊断方法。最近，引入了一种新的分生孢子ID-真菌平板（IDFP）培养基，以促进真菌的生长和取样。这项研究旨在通过与使用两个参考库的标准真菌生长培养基进行比较，评估IDFP对真菌MALDI-TOF MS的鉴定。在IDFP和Sabouraud-庆大霉素-氯霉素（SGC）琼脂上接种了总共75种丝状真菌分离株（包括32个皮肤真菌），并使用甲酸/乙腈通过MALDI-TOF MS进行了鉴定。应用了市售的Bruker库（2.0版）和公共可用的MSI Web应用程序（2018版）。对于15％的分离株，与SGC相比，IDFP的增长更快。与SGC相比，IDFP增强了MSI（属的鉴定增加16％，物种水平5％）和布鲁克库（属的鉴定增加22％和物种水平8％）的真菌鉴定性能。总体而言，Bruker文库中仅存在73％的测试菌株，而MSI文库中只有92％。无明显差异（ 对于MSI库，在IDFP和SGC之间的MALDI得分中P = 0.46），但是 在使用Bruker库时，IDFP的得分显着更高（P = 0.03），这可能是因为自Bruker数据库以来使用IDFP防止了琼脂污染是通过液体媒体创建的。IDFP是用于MALDI-TOF MS真菌鉴定的有前途的替代生长培养基，该培养基将极大地受益于优化Bruker参考库。