Methylsulfonylmethane (MSM) is an organic, sulfur-containing compound widely used as a dietary supplement to improve joint health and treat arthritic pain. An experiment was conducted to study the effects of feeding 0.05% MSM to broilers exposed to diet-induced oxidative stress on tissue MSM distribution, growth performance, oxidative stress biomarkers, and immune responsivity. A total of 528 birds were allocated to 4 dietary treatments (fresh oil-no MSM, fresh oil-MSM, oxidized oil-no MSM, oxidized oil-MSM) as provided ad libitum to 11 replicate cages of 12 birds per treatment. Blood and tissue samples were collected to analyze MSM concentrations, and oxidative stress biomarkers including concentrations of thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), total glutathione, and glutathione peroxidase (GPx) and reductase (GR) activities. Additionally, blood samples collected at day 25 were used to quantify T-cell (TC) populations using flow cytometry. Overall, MSM was quantified in all tissues and plasma samples of MSM-treated groups at all time points. Oxidized oil reduced (P = 0.006) feed intake over the 21-d feeding period, but MSM did not affect growth equally across time points. No effects (P > 0.2) of MSM or oil type were observed on TC populations. In the presence of oxidized oil, MSM reduced (P = 0.013) plasma TBARS and increased (P = 0.02) liver GPx at day 21, and increased (P = 0.06) liver GR at day 7. Irrespective of dietary oil type, groups supplemented with MSM showed higher plasma TAC at day 7 (P = 0.023), liver GPx activity at day 21 (P = 0.003), and liver GR activity at day 7 (P = 0.004) compared with groups not receiving MSM. In conclusion, 0.05% dietary MSM supplementation partially protected birds from oxidative stress but did not affect immune cell profiles.

中文翻译：

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肉鸡日粮中的甲基磺酰甲烷补充和氧化应激。

甲基磺酰甲烷（MSM）是一种有机的含硫化合物，广泛用作膳食补充剂，以改善关节健康并治疗关节炎疼痛。进行了一项实验，研究向饲喂饮食诱导的氧化应激的肉仔鸡饲喂0.05％MSM对组织MSM分布，生长性能，氧化应激生物标志物和免疫反应性的影响。共有528只家禽被分配到4种饮食处理中（新鲜油－无MSM，新鲜油－MSM，氧化油－无MSM，氧化油－MSM），每个处理均免费提供给12只鸡的11个重复笼子。收集血液和组织样本以分析MSM浓度，以及氧化应激生物标记物，包括硫代巴比妥酸反应性物质（TBARS）的浓度，总抗氧化剂能力（TAC），总谷胱甘肽，以及谷胱甘肽过氧化物酶（GPx）和还原酶（GR）的活性。此外，使用流式细胞仪将在第25天收集的血液样本用于量化T细胞（TC）群体。总体而言，在所有时间点，对MSM治疗组的所有组织和血浆样品中的MSM进行定量。氧化油在21天的采食期中减少了（P = 0.006）采食量，但MSM并未在各个时间点上均等地影响生长。没有观察到MSM或油类对TC人群的影响（P> 0.2）。在存在氧化油的情况下，MSM在第21天降低（TB = 0.013）血浆TBARS，在第7天升高（GP = 0.02）肝GPx，在第7天升高（GR = 0.06）肝GR。 MSM患者在第7天显示较高的血浆TAC（P = 0.023），在第21天显示较高的肝GPx活性（P = 0.003），在第7天显示较高的肝GR活性（P = 0）。004）与未接受MSM的组进行比较。总之，0.05％的MSM日粮补充可部分保护禽类免受氧化应激，但不影响免疫细胞谱。