This study was conducted to evaluate the effects of dietary polyunsaturated fatty acids (PUFA) sources and rosemary leaves powder (RLP) on the semen quality, fatty acid analysis, and some reproductive hormones of senescent broiler breeder roosters. Thirty-five 45-wk-old Ross breeder roosters were randomly divided into 7 groups (5 birds/group), and received following treatments including control group (basal diet), fish oil (2%), corn oil (2%), an equal (50:50%) proportion of fish oil and corn oil (50:50%), fish oil (2%) with 5 g/kg capsulated RLP, corn oil (2%) with 5 g/kg capsulated RLP, and an equal (50:50) proportion of fish oil and corn oil (50:50%) with 5 g/kg capsulated RLP of diet for 60 D, during which time their seminal characteristics were evaluated every 20 D. At the end of the trial (on day 60), semen samples were tested for determination of sperm fatty acid analysis, lipid peroxidation, and some reproductive hormones. Results showed that feeding fish oil and fish/corn oil with RLP was associated with an increase in docosahexaenoic acid (C22:6n-3) and docosatetraenoic acid (C22:4n-6) in sperm. The fish oil diet increased the proportion of n-3 fatty acids in sperm, and as a consequence, the (n-6)/(n-3) ratio also decreased (P < 0.05). RLP (5 g/kg) to the fish and fish/corn-oil (50:50%)-based diet resulted in improvement in sperm concentration, total motility (%), sperm progressive motility (%), membrane integrity, and viability in terms 0 to 60 day trial (P < 0.05). Diets and age interacted to positively affect sperm concentration and sperm membrane integrity. Also this herbal antioxidant decreased the seminal content of malondialdehyde (MDA) significantly (P < 0.05). Testosterone and LH serum levels of reproductive hormones were significantly higher in fish and fish/corn-oil with RPL (50:50%)-based diet than other groups (P < 0.05). It can be concluded that RLP as an antioxidant could remarkably improve the effects of n-3 and n-3/n-6 PUFA on sperm characteristics, seminal MDA, and hormones levels in aged breeder roosters. The susceptibility of semen to lipid peroxidation was increased in chickens fed without RLP. Future studies are needed to disclose the causal mechanisms involved.

中文翻译：

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n-3，n-6脂肪酸和迷迭香叶粉对老龄肉鸡种鸡精液参数，生殖激素和精子脂肪酸分析的改善作用。

这项研究旨在评估膳食多不饱和脂肪酸（PUFA）来源和迷迭香叶粉（RLP）对衰老肉鸡种鸡的精液质量，脂肪酸分析和某些生殖激素的影响。将35头45周大的Ross繁殖雄鸡随机分为7组（每组5只鸟），并接受包括对照组（基础饮食），鱼油（2％），玉米油（2％），鱼油和玉米油（50：50％）的比例相等（50：50％），胶囊RLP含量为5 g / kg的鱼油（2％），胶囊RLP含量为5 g / kg的玉米油（2％），并在鱼油和玉米油中按比例（50：50％）与鱼油和玉米油（50：50％）按60 D的比例封装了RLP日粮，持续60天，在此期间，每20天评估一次精油特性。试用（第60天），对精液样本进行了测试，以确定精子的脂肪酸分析，脂质过氧化和一些生殖激素。结果表明，用RLP喂养鱼油和鱼/玉米油与精子中二十二碳六烯酸（C22：6n-3）和二十二碳六烯酸（C22：4n-6）的增加有关。鱼油饮食增加了精子中n-3脂肪酸的比例，因此（n-6）/（n-3）比率也降低了（P <0.05）。以鱼和鱼/玉米油（50：50％）为基础的饮食中的RLP（5 g / kg）导致精子浓度，总运动性（％），精子进行性运动（％），膜完整性和生存力的改善在0至60天的试用期内（P <0.05）。饮食和年龄相互影响，对精子浓度和精子膜完整性产生积极影响。同样，这种草药抗氧化剂显着降低了丙二醛（MDA）的精氨酸含量（P <0.05）。在以RPL（50：50％）为基础的饮食中，鱼和鱼/玉米油中的睾丸激素和LH血清生殖激素水平显着高于其他组（P <0.05）。可以得出结论，RLP作为抗氧化剂可以显着提高n-3和n-3 / n-6 PUFA对衰老种公鸡的精子特性，精子MDA和激素水平的影响。没有饲喂RLP的鸡精液对脂质过氧化的敏感性增加。需要进一步的研究来揭示所涉及的因果机制。可以得出结论，RLP作为抗氧化剂可以显着提高n-3和n-3 / n-6 PUFA对衰老种公鸡的精子特性，精子MDA和激素水平的影响。没有饲喂RLP的鸡精液对脂质过氧化的敏感性增加。需要进一步的研究来揭示所涉及的因果机制。可以得出结论，RLP作为抗氧化剂可以显着提高n-3和n-3 / n-6 PUFA对衰老种公鸡的精子特性，精子MDA和激素水平的影响。没有饲喂RLP的鸡精液对脂质过氧化的敏感性增加。需要进一步的研究来揭示所涉及的因果机制。